摘要
目的：建立婴儿配方乳粉中V眦的高效液相荧光检测方法。方法：采用C18MG柱（250mm×4．6mm，5μm），以乙腈-磷酸缓冲盐为流动相；流速1mL／min，检测波长250／312nm。结果：VBl2的检出限为50ng／mL，线性范围在0．05035-3．2224μg／mL，回收率在86．37％-92．68％，相对标准偏差（RSD）为2．68％。结论：本方法快捷准确，适用于婴儿配方乳粉中添加的VB12的测定。
To develop an HPLC with fluorescence detection method for the determination of VB12 in infant formula powder. Method： C18 MG column （250 mm×4.6 mmμm） was used. The mobile phase consisted of acetonitrile and phosphate buffer, the flow rate was 1 mL/min. The fluorimetric detector wavelength was 250/ 312 nm. Results： The detection limit is 50 ng/mL. The linear range of the method is 0.0535-3.2224 μg/mL. The recovery is 86.37%-92.68%, RSD% is 2.68%. Conclusion： The method is fast and accurate. So it is suit to determination of VB12 in infant formula powder.
出处
《食品科技》
CAS
北大核心
2009年第5期304-307,共4页
Food Science and Technology
关键词
VB12
婴儿配方乳粉
高效液相色谱
VB12
infant formula powder
high performance liquid chromatography
