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应用原位聚合酶链反应检测淋病患者HSV-Ⅱ感染情况的报告 被引量:8

Detection of HSVⅡin the Patients with Gonorrhea by in situ PCR.
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摘要 为了解淋病患者HSVⅡ的感染情况,应用原位聚合酶链反应(ISPCR)和PCR技术对58例淋病患者进行HSVⅡ检测。结果两种方法HSVⅡDNA阳性检出率均为53.45%(31/58)。ISPCR显示,11例有皮损者中,9例阳性信号存在于上皮细胞核及胞浆,既往有疱疹皮损者6例,阳性信号均存在于细胞核,41例无明显皮损者,16例阳性,其中12例阳性信号在细胞核,4例在胞核及胞浆。结果提示,淋病患者合并有高的HSVⅡ感染率。应用ISPCR不仅可以敏感特异地检测HSVⅡ,而且可以定位。  In order to observe HSVⅡ morbidity in patients with gonorrhea,HSVⅡ in 58 patients with gonorrhes (GN) was detected by in situ polymerase chain reaction(ISPCR) and polymerase chain reaction(PCR).The results showed that the positive rates of both methods were 53.45% (31/58).Among 11 cases with lesions,9 were positive,signals were mainly found in nuclei,cytoplasma,and extracellular.The signals of 6 ceses who had the erosions before,all distributed in nuclei.Among other cases who had no obvious rash,12 cases with the signal in nuclei,4 cases'in nuclei and cytoplasma.We considered that there was high morbidity of HSVⅡ in the GN.It suggested that ISPCR is a sensitive and specific method which can locate and contribute to clinical diagnosis and treatment.
出处 《中国皮肤性病学杂志》 CAS 北大核心 1998年第2期76-77,共2页 The Chinese Journal of Dermatovenereology
关键词 淋病 HSV-Ⅱ 聚合酶链反应 检测 Gonorrhea Herpes simplex virusⅡ Polymerase chain reaction In situ PCR
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