摘要
目的:探讨不同温度对于地塞米松(DEX)介导的下丘脑神经元内游离钙离子浓度([Ca2+]i)下降幅度的影响。方法:使用原代培养的Wistar新生大鼠下丘脑神经元,用Fluo-3/AM钙荧光探针标记细胞内游离Ca2+,分别在18～19℃、21～22℃、24～25℃三种温度条件下,以10-6MDEX刺激D′hanks液中的神经元,在激光共聚焦显微镜下连续观察不同温度下神经元内钙荧光变化。结果:在24～25℃条件下,10-6MDEX刺激使培养的下丘脑神经元细胞内[Ca2+]i迅速减少20.6%;在21～22℃时减少约36.8%;而18～19℃时则减少约61.7%。结论:在一定范围内,DEX介导的下丘脑神经元[Ca2+]i下降幅度随着温度的降低而增大。
Objective：To investigate the effects of different temperatures on 10-6 M dexamethasone （ DEX）- induced decreases of [Ca^2＋]i. L Methods ： The hypothalamic neurons from 2 - 3 days old Wistar neonatal rats were primarily cultured, and then the intracellular Ca^2＋ in these cells were marked with calcium fluorescent probe of Fluo - 3/AM. Under the temperature of 18 - 19℃ ,21 -22℃ ,24 - 25℃, 10 -6 M DEX was used to stimulate the hypothalamic neurons in D＇ hanks solution and the fluorescent values were recorded continuously under confocal laser scanning microscope. Results ： Under 24 - 25℃, the exposure of 10 -6 M DEX induced 20.6% decrease of [Ca^2＋]i in the cultured hypothalamic neurons within 2 seconds. Under 21 - 22 ℃ ,the DEX induced 36.8% decrease of [Ca^2＋]i and under 18 - 19 ℃ ,61.7% decrease of [Ca^2＋]i occurred. Conclusion：Within a certain range, the descending amplitude of [Ca^2＋]i in the cultured hypothalamic neurons induced by 10-6 M DEX increases with the decrease of temperature.
出处
《西南国防医药》
CAS
2009年第5期465-467,共3页
Medical Journal of National Defending Forces in Southwest China
基金
国家重点基础研究发展规划项目(2005CB522602)
