摘要
对籼型杂交水稻B优827苗期进行不同PEG-6000浓度的水分胁迫处理，研究不同水分胁迫下丙二醛（MDA），O2^-产生速率，超氧化物歧化酶（SOD）和过氧化物酶（POD）同功酶的变化。MDA和O2^-产生速率在轻度水分胁迫下随着胁迫时间的延长，其含量逐渐增加。重度水分胁迫下变化与轻度胁迫下变化相似，但是其含量的上升速度明显比轻度快。对中度和重度水分胁迫下0—4d的叶片进行同功酶电泳比较，发现7．5％PEG处理下，第2天开始出现SOD、POD新条带。20％PEG处理下，第4天出现SOD新条带，POD在胁迫的第1天就出现了新同功酶条带，并随着胁迫时间的增加其同功酶条带有增有减。
Hybrid rice B You 827 seedlings were treated by PEG-6000 of different concentration to investigate the variations in MDA, O2^- , superoxide dismutase （SOD）and peroxidase （POD） isozymes. The results showed that, in light water stress, the contents of MDA and O2^- increased slowly with the continuance of the stress. In severe water stress ,the contents of MDA and O2^- increased faster than that in light water stress with the continuance of the stress. The isozymes under 7.5 % and 20 % PEG for four days were detected out. new isozymes of SOD and POD began to appear at the second days of 7.5 % PEG. Under 20 % PEG, new SOD isozymes were detected at the fouth day, and POD new isozymes were detected at the first day,which were increased or reduced with the continuance of wate stress.
出处
《西南农业学报》
CSCD
北大核心
2009年第2期286-289,共4页
Southwest China Journal of Agricultural Sciences
基金
四川省"十一五"育种攻关项目(2006-YZGG-1)
