摘要
目的:分析药用植物华南忍冬5个居群的遗传多样性和分化程度。方法:应用随机扩增多态性DNA(RAPD)技术扩增可分析位点。结果:10条随机引物扩增出100个可分析位点,多态位点百分率(PPL)为87.00%。经POPGENE分析发现,华南忍冬居群平均水平的多态位点百分率为58.86%,Ne i′s基因多样性(H)为0.2647,Shannon′s信息指数(I)为0.4022,具有较高水平的遗传多样性。居群间遗传分化程度较高(Gst=0.3839);地理距离与遗传距离之间具有显著相关性(r=0.8504,P=96.80%),表明地理隔离效应是导致居群间遗传分化的重要因素。根据遗传距离(POPGENE软件)和遗传相似系数(NTSYS-pc软件)的UPGMA聚类分析,罗浮山居群(LFS)和新兴居群(XX)先聚在一起,再与南宁居群(NN)聚成一类,而徐闻居群(XW)和海口居群(HK)聚成另一类。结论:基于华南忍冬的物种保护与资源利用,建议加强现有自然居群的就地保护,促进居群的自然更新;建立种质资源库,从中选育优良品系用于药材的规范化种植。
Objective: To analyze the genetic diversity and differentiation degree of five Lonicera confusa populations.Methods: Random Amplified Polymorphic DNA(RAPD) markers were perfomed to amplify the discernible loci.Results: A total of 100 loci were obtained for all populations using ten primers,87.00% of which were polymorphic(PPL=87.00%).Analyzed by POPGENE,the average percentage of polymorphic loci was 58.86%.Nei′s genetic diversity(H=0.2647) and Shannon′s information index(I=0.4022) indicated that Lonicera confusa had a higher level of genetic diversity.The high value of differentiation(Gst=0.3839) showed that geographic isolation strongly influenced genetic differentiation among populations and there was a significant correlation between genetic distance and geographic distance(r=0.8504,P=96.80%).The clustering analysis was performed using softwares POPGENE and NTSYS-pc.Five populations were divided into 2 clusters,named cluster 1(LFS、 XX and NN) and cluster 2(XW and HK).Conclusion: Management strategies were proposed for species conservation and resource utilization of Lonicera confusa,including the protection from human disturbance to facilitate natural regeneration,construction of germplasm resources and cultivation of plants collected from different populations,in order to select elite lines for good agricultural practice(GAP) of medicinal materials.
出处
《中药材》
CAS
CSCD
北大核心
2009年第4期485-490,共6页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金项目(5006633)
广东省中医药管理局科研项目(1040102)
华南农业大学校长基金科研项目(4600-K03156)
