摘要
用不同剂量(200~500 Gy)的60Co-γ射线辐射日本结缕草Zoysia japonica干种子,通过对辐射后干种子的发芽和幼苗生长情况进行了室内外观测,结果表明:低剂量辐射处理促进日本结缕草干种子的萌发,提高其发芽率,随着辐射剂量的增加,发芽率逐渐降低,临界辐射剂量(剂量率为1.0 Gy/min)为450 Gy;田间出苗率临界辐射为250 Gy,低于临界辐射剂量时,随辐射剂量的增大,田间出苗率增加,高于临界辐射剂量时,随辐射剂量增大,田间出苗率降低;不同的剂量处理,干种子发芽后根系和幼苗的生长差异显著(P<0.05),在主根长度、幼苗高度和幼苗鲜质量方面表现为先促进后抑制,临界辐射剂量分别为400、300和250Gy;根据辐射剂量与相对出苗率的关系折线图可以初步确定,日本结缕草干种子辐射育种的半致死剂量为480 Gy。
The dry seeds of Zoysia japonica were irradiated by ^60Co-γray at dosage of 25-500 Gy. The germination of seeds and growth of seedlings were investigated both in laboratory and field. The results indicated that irradiation of low dosage could promote seed germination and increase germination rate. But the germination rate decreased with the increasing of irradiation dosage, the critical dosage （dosage rate was 1.0 Gy/min） is 450 Gy; the critical dosage for field germination rate was 250 Gy, when the dosage below the critical was applied, the field germination rate increased with the increasing of the irradiation dosage, and decreased when the dose was over the critical; significant difference（P〈0.05） observed in taproot and seedling growth when the dry seeds were irradiated at different dosage; the length of taproot, height and fresh weight of seedling were all promoted firstly and declined later, their critical dosage was 400 Gy, 300 Gy and 250 Gy, respectively. The half lethal dosage of Z. japonica dry seeds was 480 Gy based on correlated broken line graph between irradiation dosage and relative germination rate.
出处
《草业科学》
CAS
CSCD
北大核心
2009年第5期155-160,共6页
Pratacultural Science
基金
国家"863"课题"优质抗逆草坪草新种质培育技术研究"(2004AA244050)
