摘要
Carnoy′s fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially those located in intestinal mucosa(MMC)and in thymus medulla(TMMC). Both toluidine blue and Alcian blue were the excellent stains generally,but Alcian blue stained more porcine mast cells than did toluidine blue( P <0 01). Staining with toluidine blue of a wide pH range(from 0 1 to 7 0)showed that porcine mast cells were not very pH dependent,but the dye at pH 0 5 seemed to have the strongest affinity for all mast cells in pigs and it was also suitable for bovine and ovine mast cell staining. In the three species,unlike in rodents,the Alcian blue method did not distinguish between mast cells in the intestinal mucosa(MMC)and those in the connective tissue of the intestinal submucosa,tongue and skin(CTMC). Porcine CTMC,but not MMC,fluoresced strongly when stained with berberine sulphate or with a mixture of berberine sulphate and acridine orange. It suggested that porcine CTMC contained heparin proteoglycan.
研究证实Ｃａｒｎｏｙ液和中性缓冲福尔马林溶液（ＮＢＦ）是猪、牛及绵羊肥大细胞的优良的固定液，ＮＢＦ虽能很好地保存牛和绵羊的肥大细胞，但却阻断了猪的大多数肥大细胞、特别是肠粘膜肥大细胞（ＭＭＣ）及胸腺髓质肥大细胞（ＴＭＭＣ）对碱性染料的着染力。甲苯胺蓝及阿尔辛蓝均为动物肥大细胞的优良染料，但阿尔辛蓝能使更多的肥大细胞着染（Ｐ＜００１）。不同ｐＨ的甲苯胺蓝染色试验，显示猪肥大细胞对染液ｐＨ的要求虽不十分严格，但ｐＨ０５时似乎着染力最强，且也适用于牛及绵羊肥大细胞的染色。与啮齿动物不同的是，阿尔辛蓝染色并不能区分这３种动物的ＭＭＣ及结缔组织肥大细胞（ＣＴＭＣ）。当采用硫酸小蘖硷或硫酸小蘖硷与吖啶橙混合液染色时，猪ＣＴＭＣ可显示出强荧光而ＭＭＣ则无反应，证明猪ＣＴＭＣ含肝素蛋白多糖。
