摘要
建立了重要医学真菌──肺微小根毛霉的原生质体生成系统。采用溶细胞酶以及蜗牛酶与纤维素酶联合作用的方式获得了大量原生质体，处于指数生长期、生长旺盛的幼龄菌丝对破壁酶比生长缓慢或老龄菌丝更敏感；作为渗透压稳定剂，无机盐类要好于有机物；原生质体的较适生成温度为31℃。
A Protoplashng tecboque has been den'eloped for the medically imPOrtant fungusAnizomucor pusl'llus isolated by Prof. Chen Shi--ping, in other for itS genehc research andepidendological fingerprinhng. gogh yield of protoplast from Rpusillus was obscened by using theconnnereial enzyme, Lyhcase and combined enzyme system, the effect of which is much better thanusing only one enzyme. Achvely growing mycelia of R pusillus were more suscephble tOProtOPlashng enzymes than slow--growing or older myceliec As osmohc stabilizef, (NaO, KCI)ino4edc saltS were more effechve for the protOPlastS formation than orgedc material. The optimaltemperathe is 3it for the protoplast produchon of R pusillus.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第1期27-29,共3页
Microbiology China
