摘要
目的：测定致倦库蚊幼虫、具抗性蚊幼虫可溶性蛋白的组分，并分析、评价其特异性蛋白区带。方法：对正常和具抗性的Ⅲ龄、Ⅳ龄初幼虫的可溶性蛋白提取物进行聚丙烯酰胺凝胶电泳分析。结果：正常蚊幼虫主要有１２条蛋白区带，其中５５ＫＤａ和５０ＫＤａ为其特有；对马拉硫磷及溴氰菊酯具抗性的蚊幼虫，分别有１５和１７条蛋白区带，并发现６６ＫＤａ蛋白为特异性蛋白分子。结论：具抗性的蚊幼虫所特有的６６ＫＤａ蛋白分子可能为导致蚊幼虫对杀虫剂产生抗性的特异性蛋白。
To determine protein component, specific protein molecular from normal larvae, pesticidesresistant larvae of Culex fatigans and evaluation of the specific insecticideresistance protein bands. Soluble proteins of the late Ⅲinstar and the early Ⅳinstar larvae of both normal and pesticidesresistant mosquito were prepared. Protein component bands were tested by SDS-PAGE.The normal larvae of Culex fatigans contained mainly 12 protein bands, the 55 and 50KDa were particular protein molecular, and insecticide-resistance larvae to Malathion and Deltamethrin present at 15 and 17 protein bands respectively. The 66KDa that stronger and clearer protein bands was recognized by resistance larvae only. It could be one of the main proteins to cause mosquito larvae resistance.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1998年第1期14-16,共3页
Chinese Journal of Vector Biology and Control
