摘要
目的对传统的增生性瘢痕动物模型进行改进，为增生性瘢痕发病机制的研究建立更实用的动物模型。方法将20只裸鼠随机分成观察组和对照组，每组10只。观察组裸鼠背部皮下移植人全厚皮肤，皮片存活后用加热的铜柱造成深Ⅱ度烧伤；对照组裸鼠如同观察组移植人全厚皮片，但未予热烧伤，观察皮片存活、创面愈合与瘢痕增生情况。结果观察组裸鼠存活9只，移植存活皮片与正常成人皮肤相比，无明显差异；烧伤后有8只可见明显、持续的瘢痕增生，其外观和组织学特点与人体增生性瘢痕相似，组织学观察可见丰富胶原纤维和炎症浸润。对照组存活8只，皮片干痂脱落后，有6只出现类似瘢痕样增生。结论与以往模型相比，改进后模型的组织来源更可靠，皮片存活率及瘢痕复制率更高，建立周期更短，且瘢痕增生明显，可用于观察创面愈合至瘢痕形成的全过程，因此是一种较理想的研究增生性瘢痕的动物模型。
Objective To improve the animal model of hypertrophic scars for studying their pathology. Methods Twenty nude mice were divided randomly into two groups of 10. In group one every mouse received a full thickness piece of human skin grafted into the subcutaneous tissue of their back. After the graft had survived 14d after transplantation, a burn was caused on the graft with a heated copper cylinder. The mice in the second group received the transplant but no burn. The status of the grafts and scars was observed, and histological examinations of the grafts were performed. Results Nine mice in group one survived, of which eight developed obvious and persistent hypertrophic scars after the burns. These were hard and rose above the surrounding skin. Eight nude mice survived in group two, of which only six developed hypertrophic scar analogues after transplantation. Histological examinations showed abundant collagen deposition and inflammatory infiltration in all scars. Conclusion The similarity, repeatability, certainty, and survival rate of this improved hypertrophic scar model are superior to those reported previously. This improved animal model can induce obvious and persistent scarring that can be used to observe the whole procedure of hypertrophic scarring, so it is a useful tool for studying hypertrophic scars.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2009年第5期289-291,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金项目（30672214）
关键词
烧伤
瘢痕
模型
动物
Burns
Scarring
Animal models
