摘要
[目的]通过对编织聚乙烯醇(polyvinyl alcohol,PVA)材料的生物相容性和力学性能的分析,初步探讨使用该材料构建组织工程前交叉韧带支架的可行性。[方法]将体外分离培养的人前交叉韧带细胞和NIH3T3细胞分别种植在编织构建支架材料上进行立体培养,观察细胞在支架材料上的生长情况;然后使用电子拉力机对支架材料进行力学性能测试并进行分析。[结果]细胞在支架材料上生长良好,并分泌细胞外基质;支架材料的最大负荷、最大应变和极限抗张强度分别达169.78±9.18N、11.67%±1.38%、52.21±2.88MPa。[结论]该支架材料具有优良的生物相容性和一定的力学性能,有望通过改进力学性能后成为一种较理想的组织工程前交叉韧带支架材料。
[ Objective] To investigate the feasibility of the fabrication of tissue-engineered anterior cruciate ligament （ACL） in vitro by studying biocompatibility and mechanical property of the braided polyvinyl alcohol （PVA） materials. [ Method] Firstly, human ACL cells and NIH3T3 cells were isolated, expanded in vitro and seeded onto the surface of the braided PVA scaffold materials, the adhesion, proliferation and three-dimensional growth of cells on the scaffold were observed by SEM. Secondly, the biomechanical properties of the braided PVA scaffold materials were measured with electro-biomechanical machine. [ Result] The braided PVA scaffold materials had no cytotoxicity, ACL and NIH3T3 cells adhered, grew and proliferated well both on the surface and in the holes of the braided PVA scaffold materials. The maximum load, the maximum strain and ultimate tensile stress of the braided PVA scaffold materials respectively were 169.78 ±9. lgN, 11.67 ±1.38% and 52.21 ±2.88MPa. [ Conclusion] The braided PVA scaffold materials possess good biomechanical properties and biocompatibility, it may become an ideal biomaterial for fabricating tissue-engineering ACL if the biomechanical properties can be improved.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2009年第10期769-773,共5页
Orthopedic Journal of China
关键词
聚乙烯醇
支架材料
编织
生物相容性
力学性能
膝前交叉韧带
polyvinyl alcohol（PVA）
scaffold material
braiding
biocompatibility
mechanical property
knee
anterior cruciate ligament（ACL）
