摘要
目的:检测缺血性股骨头坏死患者血清同型半胱氨酸(HCY)、超敏C反应蛋白(hs-CRP)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)浓度值,探讨HCY、hs-CRP在缺血性股骨头坏死发病机制中作用。方法:实验分为4组,非股骨头坏死正常组(N组)、股骨头坏死Ⅰ期组(N1组)、股骨头坏死Ⅱ期组(N2组)、股骨头坏死Ⅲ期组(N3组)。双抗体夹心ELISA法测定hs-CRP、IL-1β、TNF-α,采用荧光标记免疫检测法检测血清HCY水平,生物化学法测定血脂。结果:N1、N2、N3组和N组比较,HCY、hs-CRP、IL-1β、TNF-α浓度明显升高(P<0.01);N3组和N1组比较,HCY、hs-CRP、IL-1β、TNF-α浓度明显增高(P<0.01)。结论:HCY、hs-CRP参与缺血性股骨头坏死的发病机制。HCY参与缺血性股骨头坏死发生发展的炎性反应过程,炎症反应可能是缺血性股骨头坏死的促发因素。
Objective To detect the serum levels of homocysteine （HCY）, high-sensitive C-reactive protein （hs- CRP）, interleukin-1β （IL-1β）, and tumor necrosis factor-α （TNF-α） in patients with avascular necrosis in femoral head, and to explore the role of HCY and hs-CRP in the pathogenesis of it. Methods Patients with avaseular necrosis in femoral head were divided into 4 groups, normal group （group N）, stage-one of necrosis in femoral head group （group N1 ）, stage- two of necrosis in femoral head group （group N2）, and stage-three of necrosis in femoral head group （group N3）. Serum levels of hs-CRP, interleukin-1β （IL-1β）, and TNF-α were measured by enzyme linked immunosorbent assay, HCY was measured by immunofluorescenee, and blood lipid was detected by biochemical method. Results The serum levels of HCY, hs-CRP, IL-1β, TNF-α were significantly higher in group N1, N2, and N3 than in group N （P 〈 0.01）, and were significantly higher in group N3 than in group N1 （P 〈 0.01）. Conclusions HCY and hs-CRP may participate in the pathogenesis of avascular necrosis in femoral head. HCY may play an important role in the process of inflammatory reaction, which may be a precipitating factor in avascular necrosis in femoral head.
出处
《实用医学杂志》
CAS
北大核心
2009年第9期1415-1417,共3页
The Journal of Practical Medicine
基金
重庆市自然科学基金资助项目(编号:cqsf07k012)
关键词
股骨头坏死
同型半胱氨酸
超敏C反应蛋白
Femur head necrosis
Homocysteine
High-sensitive C-reactive protein
