摘要
目的:观察支原体肺炎(MPP)患儿外周血免疫球蛋白、T淋巴细胞亚群以及细胞因子的含量变化,并探讨其在MPP发病机制中的作用。方法:采用流式细胞技术检测43例MPP患儿和30例正常对照组儿童外周血T淋巴细胞亚群CD3+、CD4+、CD8+,并用速率散射比浊法检测血清IgG、IgA和IgM含量,采用ELISA检测血清干扰素-γ(IFN-γ)、白介素(IL-2、IL-4和IL-6)水平。结果:MPP患儿外周血CD3+、CD4+T细胞百分率分别为61.45±6.75和33.52±5.81,较正常对照组68.28±7.34和38.71±6.29显著降低(P<0.05),CD8+T细胞和CD4+/CD8+比值较对照组无显著差别(P>0.05);MPP患儿血清免疫球蛋白与正常对照组比较,IgG和IgA均无明显差异,IgM较对照组增高(P<0.05);MPP患儿IFN-γ、IL-4、IL-6血清水平以及IFN-γ/IL-4比值较正常对照组明显增高(P<0.05),而IL-2水平低于正常对照组(P<0.05)。结论:MPP患儿存在免疫功能减低,免疫调节紊乱,辅助性T细胞亚群(Thl/Th2)失衡,并以Thl型细胞介导的免疫反应占相对优势状态。
Objective:To observe the changes of peripheral blood T lymphocyte subsets,the immunoglobulin content and in children with mycoplasma pneumoniae pneumonia(MPP),and to explore the possible pathogenesis of MPP.Method:Forty-three pediatric patients with Mycoplasma pneumoniae(MP)infection during their acute stage and 30 healthy children served as control were involved in this study.The percentages of T lymphocyte subsets(CD3+、CD4+、CD8+)in the peripheral blood were determined by flow cytometry.The levels of IgG,IgA and IgM were measured by velocity scatter colorimetry.Results:The percentages of CD3+ and CD4+ T lymphocyte in MPP group were significantly lower than those in control group(P<0.05).There was no significant difference in the percentages of CD8+ T lymphocyte,CD4+/CD8+,IgG and IgA between the MPP group and control group(P>0.05).The levels of IFN-γ,IL-4 and IL-6 of MP infected children were obvious higher than those of control(P<0.05).Conclusion:There was an unbalanced cell mediated immunity with a tendency toward Th1 existed in children with MPP infection.
出处
《微循环学杂志》
2009年第2期38-40,共3页
Chinese Journal of Microcirculation
