摘要
[ Objective] The study aimed to explore the effects of magnetic treatment on the quality of chicken semen stored at low temperature. [ Method] 5 ml fresh chicken semen was divided into five groups equally, each of which was diluted at the volume ratio 1:4. With the group without magnetic treatment as the control, the other four groups were magnetized for 6, 12, 24 and 48 min in the self-made magnetizer, respectively. Subsequently, all the five groups were stored at 2 -4 ℃, and the sperm motility, survival time, survival index and deformity rate were observed regularly. [ Result] Comparing with the control group, the magnetic groups showed higher sperm motilities and effective survival indices as well as lower deformity rates. The effective survival index of the group magnetized for 24 min was the highest and increased by 7.75% in con- trast to the control. [ Conclusion] Magnetic treatment can effectively enhance the quality of chicken semen stored at low temperature.
1材料与方法1.1材料 选40只健康、精液品质良好的伊沙种公鸡供采精用。精液简易磁化装置:N、S两极磁场大小范围为56mT左右,两磁极间距为3cm,磁化瓶置于两磁极正中间。显微镜、烘干箱、灭菌锅、滴管、集精杯、血球分类计数器、电冰箱、载玻片、盖玻片等。药品及稀释液:青霉素、硫酸链霉素、甘农一号稀释液。