摘要
目的检测PTEN和磷酸化Akt在婴幼儿血管瘤不同时期的表达情况,分析二者的表达相关性,探讨其在婴幼儿血管瘤发生发展过程中的作用及意义。方法应用免疫组化SP法检测婴幼儿血管瘤增生期、消退期,血管畸形及正常皮肤中的PTEN和磷酸化Akt蛋白表达情况,利用计算机图像分析技术测量其平均光密度。结果PTEN在增生期血管瘤内皮细胞的表达低于消退期、血管畸形及正常皮肤(P<0.01);磷酸化Akt在增生期血管瘤内皮细胞的表达高于消退期、血管畸形及正常皮肤(P<0.05);消退期血管瘤、血管畸形与正常皮肤间PTEN、磷酸化Akt的表达差异无统计学意义(P>0.05);PTEN与磷酸化Akt在婴幼儿血管瘤组织中的表达呈负相关(r=-0.7192,P<0.05)。结论PTEN与磷酸化Akt可能在婴幼儿血管瘤增生过程中起了一定的作用。
Objective To determine expressions of PlaN and p-Akt in different stages of infantile hemangioma, and to explore the correlation between PTEN and p-Akt and approach significance of PTEN and p-Akt in the occurrence and development of infantile hemangioma. Methods Expressions of PTEN and p-Akt were determined by SP immuno histochemical technique in cases of proliferating hemangiomas, involuting hemangiomas, vascular malformations, and normal skins. Mean optical density was measured by an image analysis system. Results Expression of PTEN in proliferating hemangiomas was lower than in the other groups （ P 〈 0.01 ）. Expression of p-Akt in proliferating hemangiomas was higher than in the other groups （ P 〈 0.05 ）. No statistical difference was found in expressions of PTEN and p-Akt among involuting hemangioma, vascular malformation and normal skins. Expression of P＇FEN was negatively correlated with expression of p-Akt （ r = - 0.719 2, P 〈 0.05）. Conclusions and p- Akt may play important roles in the proliferating phase of infantile hemangioma.
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第5期95-98,共4页
Journal of Shandong University:Health Sciences
关键词
血管瘤
基因
PIEN
AKT
Hemangioma
Phosphatase and tensin homologue deleted on chromosome 10
Akt
