摘要
目的:观察龙牙楤木总皂苷对缺血再灌注损伤心肌粘附分子sVCAM-1表达及MDA、GSH-PX的影响。方法:可逆性冠脉左前降支结扎缺血45min再灌注6h复制MI/R模型,Wistar雄性大鼠30只,随机分成模型组、龙牙楤木组、波立维组,每组10只。HE染色观察心肌病理学变化,检测血清LDH含量,测定心肌MDA、GSH-PX活性,ELISA法测定血清sVCAM-1的表达。结果:与模型组比较,龙牙楤木总皂苷、波立维均能显著降低缺血再灌注大鼠血清LDH含量,显著升高GSH-PX的活性,升高MDA含量,缺血45min再灌注6h血清sVCAM-1有明显表达,各组之间无统计学差异。结论:龙牙楤木总皂苷对MIRI有保护作用,可能是通过减少MDA含量、升高GSH-PX活力,对抗氧自由基对心肌细胞的毒害作用,减轻心肌细胞的损伤而实现的。
Objective： To investigate the effects of Aralosides on expression of inflammatory molecule sVCAM-1 and the activity of Malcic Dialdchyde （MDA） ＆ Glutathione Peroxidase （GSH-PX） in rats after myocardial ischemia/reperfusion （MICR） injury. Methods： A model of myocardial ischemia 45min followed by 6h reperfusion was made by reversibly ligating coronary left descending artery. 30 Wistar rats were divided randomly into 3 groups ： Ichemia reperfusion Group, Aralosides Goup, Plavix Group. HE staining was used to observe the pathological changes of myocardial issues. The activity of MDA and GSH-PX was measured , too. ELISA was used to determine sVCAM-1 expression in myocardium. Results： In rats treated by Aralosides or Plavix, the lactate dehydrogenase LDH） activity was significantly decreased. Meamwhile , Aralosides and Plavix raised the activity of Malcic Dialdchyde （MDA） ＆ Glutathione Peroxidase （GSH- PX） . sVCAM-I expression was detected in myocardium after ischemia 45min followed by 6h reperfusion. In rats treated by Aralosides or Plavix, sVCAM-1 expression remained unchanged. Conclusion： Aralosides could protect myocardium from inflammatory injury during MI/R. It had the actions of decreasing MPO activity.
出处
《中国现代中药》
CAS
2009年第5期30-32,61,共4页
Modern Chinese Medicine
基金
国家自然基金课题(30572461)
