摘要
以"红香椿一号"的茎段为外植体进行植物组织培养,采用四因素三水平正交设计法试验筛选出生根、快繁的适宜培养基。以不同培养基的生根数为鉴定指标,筛选出适宜红香椿一号茎分化苗生根的培养基,以不同培养基的芽增殖数为鉴定指标,筛选出适宜"红香椿一号"茎分化丛生芽的培养基,进而掌握不同生长素之间的适宜配比。通过试验研究表明:在9个培养基中生根最佳的培养基是(MS+6-BA0.1mg/L+NAA1.0mg/L+IBA1.0mg/L+ZT0.2mg/L);增殖适宜的培养基是(MS+6-BA1.0mg/L+NAA0.1mg/L+IBA0.1mg/L+ZT0.1mg/L)。
We sifted out suitable medium of rooting and rapid reproduction by adopting methods on experiment with four-factor three-level orthogonal design using stems of Toona sinensis （A. Juss.） Roem as explants for plant tissue culture. We also sifted out the suitable medium for rooting of the seedings from differentiation of stem of Toona sinensis （A. Juss.） Roem. According to the rooting number in different medium and we sifted out the suitable medium for budding of thicket from differentiation of stem of Toona sinensis （A. Juss.） Roem and then grip the suitable ratio among the different auxins. The results showed that： the medium of （MS＋6-BA 0.1 mg/L＋NAA 1.0 mg/L＋IBA 1.0 mg/L＋ZT 0.2 rag/L） is optimal for rooting in the nine mediums and the medium of MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L＋IBA 0.1 mg/L＋ZT 0.1 mg/L is the best one of proliferation medium.
出处
《中国农学通报》
CSCD
北大核心
2009年第10期116-119,共4页
Chinese Agricultural Science Bulletin
基金
商丘市科技发展计划项目"优质速生红香椿1号的快繁及应用研究"(20051038)
关键词
红香椿
培养基
组织培养
正交试验
Toona sinensis （A. Juss.）Roem, medium, tissue culture, orthogonal experiment
