摘要
目的以化学成分指纹图谱为检测手段,探索丹参的常用炮制方法对其化学成分变化的影响,以及丹参饮片在汤剂中不同提取次数下化学成分的溶出特点。方法采用HPLC色谱指纹图谱技术和标准化学对照品对比分析的方法,研究丹参炮制前后化学成分的变化及汤剂可溶出成分与药材成分的差异。结果丹参药材及净制饮片中只有原儿茶醛,未发现原儿茶酸,而在酒炙饮片中发现了原儿茶酸,且原儿茶醛相对含量下降;丹参饮片的脂溶性成分在汤剂中不宜溶出,水溶性成分提取2次,即可大部分提取出来。结论丹参饮片中的脂溶性成分难以进入到汤剂中,说明丹参在汤剂中使用、产生疗效的物质基础化学成分应该是水溶性成分。
Objective To investigate the influence of common preparation methods of Radix Salviae Miltiorrhizae on the changes of its chemical compositions by applying the fingerprint chromatogram, and its dissolution characteristics for different times of extraction in the decoction of Radix Salviae Miltiorrhizae. Method The changes of chemical compositions and difference between extractable compositions and original compositions of CHR were compared before and after the preparation by applying HPLC fingerprint chromatogram and the control and analytic method of chemical standard samples. Result There were only protocatechualdehyde in the material and the slices of Radix Salviae Miltiorrhizae. There was no protocatechuic acid observed, but which was found out in the stirfrying slices with wine and then the relative content of protocatechualdehyde decreased at the same time. The liposoluble compositions of the slices was dissolved out uneasily in the decoction, while the most of watersoluble compositions were extracted out after extracting for two times. Conclusion It is difficult for the liposoluble compositions of slices of Radix Salviae Miltiorrhizae to dissolve into the decoction, which indicates that the effective material base （chemical decoction should be its water-soluble compositions.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第4期256-258,共3页
Journal of Beijing University of Traditional Chinese Medicine
关键词
丹参
炮制
汤剂
化学成分
高效液相色谱
Radix Salviae Miltiorrhizae
processing
compositions） of Radix Salviae Miltiorrhizae in the decoction
chemical compositions
HPLC
