摘要
目的:探讨TNF-A-863与CGRP979基因多态性与重度慢性牙周炎易感性的关系。方法:收集100例重度慢性牙周炎患者和118例健康对照组的颊黏膜拭子并抽提DNA,应用PCR-LDR方法检测TNF-A-863与CGRP979基因型,用计算机软件统计分析患者和对照组间基因型分布的差异。结果:TNF-A-863在重度慢性牙周炎组中TNF-A-863"A/C"杂合子占优势,在健康对照组中TNF-A-863"C/C"纯合子基因型为主,TNF-A-863基因型分布在两组间的差异有统计学意义(P<0.05)。CGRP979在重度慢性牙周炎患者中"A/G"基因型明显占多数,两组间基因型分布有统计学意义(P<0.05)。结论:TNF-A-863基因多态性与重度慢性牙周炎有相关性;CGRP979等位基因"A/G"杂合子基因型可能是重度牙周炎的易感因素。
Objective：To investigate the relationship of TNF-A-863 and CGRP979 gene polymorphisms with the susceptibility to severe chronic periodontitis in Chinese. Methods： Buecal swabs were collected from 100 adult patients with severe chronic periodontitis and 118 healthy adult controls. DNA was extracted from each subjects of the two groups. PCR-LDR technique was used to identify the genotypes of TNF-A-863 and CGRP979. The difference in the genotypes between the two groups was analyzed by statistics software. Results： The genotype of TNF A-863 was mainly TNF A-863 A/C in patients with severe chronic periodontitis and TNF-A-863 C/C in healthy controls. There were significant differences in TNF-A-863 distribution between the two groups（P〈0.05）. We also found that there were significant differences in genotype distribution of CGRP979 between the two groups （P〈0.05） ,with A/G predominating in patients with severe chronic periodontitis. Conclusion： TNF-A-863 polymorphism is associated with severe chronic periodontitis; A/G of the CGRP979 loci might be a factor for severe chronic periodontitis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2009年第5期541-544,共4页
Academic Journal of Second Military Medical University
基金
上海市自然科学基金(06ZR14123)
全军"十一五"面上项目(06ZR14123)~~
