摘要
目的观察大黄酸抗猪血清诱导的大鼠肝纤维化的效应并探讨其作用机制。方法用猪血清腹腔内注射复制免疫损伤性肝纤维化模型,造模同时给予大黄酸口服作为肝纤维化防治组;免疫攻击16周后处死动物。行肝组织病理分析,免疫组化法检测肝内结缔组织生长因子(CTGF)、TGF-β1表达。结果16周后模型组大鼠形成典型的肝纤维化,肝内CTGF与TGF-β1表达均显著增强,以大黄酸防治的大鼠,肝纤维化程度明显减轻,CTGF和TGF-β1表达的阳性指数下降。结论大黄酸能有效地减轻猪血清诱导的肝纤维化大鼠的肝损伤及纤维化程度,其机制可能与抑制TGF-β1和CTGF表达有关。
Objective Adopt immunological hepatic fibrosis induced by porcine serum in rats. To investigate the anti fibrotic and hepatocyte- protective effects of rhein （RH） and to elucidate its mechanism. Methods All rats were given subcutaneous injection of pig serum after 16 weeks. Degrees of fibrosis were detected by histopathology under optical microscopy. The expressions of CTGF,TGF-β1 were measured by immunochemical techniques. Results The model group had shown typical hepatic fibrosis, the expressions of Transforming growth factor- β1 ,Connective tissue growth factor increased obviously, rehin-treated group could strikingly reduced the collagen area and the expressions of Transforming growth factor-β1.Connective tissue growth factor was apparently superior to model contrast group. Conclusion Rehin could improve liver functions and slow down the progression of hepatic fibrosis. The mechanism of such effects may due to inhibiting express of TGF-α and CTGF.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第10期1204-1206,共3页
Chongqing medicine
