摘要
[目的]建立高效液相色谱法测定骨质宁搽剂中盐酸小檗碱(C20H18ClNO4)含量的方法。[方法]C18色谱柱,流动相为乙腈—50mmol/L,NaH2PO4水溶液(磷酸调PH=2.5)(25:75),流速1.0ml/min,室温,UV348nm检测。[结果]盐酸小檗碱在0.106～0.530μg范围内线性关系良好(r=0.9999);方法精密度RSD为0.7%。4批样品盐酸小檗碱的含量分别为19.11、18.81、18.83、18.92mg/100mg。[结论]方法专属性强、简便、准确;为该药提供了切实可行的质控方法。
[Objective]To develop an HPLC method for the determination of berberine hydrochloride in GuZheNing liniment. [Methods]The chromatographic system consisted of Dimonsil C18 column and mobile phase of acetonitrile--50mmol · L^-1 sodium dihydrogen phosphate（adjusted PH to 2.5 with H3 PO4 ）（25.-75）. The flow rate was 1.0 mL · min^-1, and the detective wavelength was at 348 nm. [Results]The calibration curve was linear in the range of 0. 106-0. 530 μg（n=5）. The method was proved to he repeatable with RSD 0.7% （n = 6）. 4 batches of sample were analyzed and the Berherine Hydroehloride contents were 19.11,18.81,18.83,18.92mg/100mg,respeetively. [Conelusion]The method is selective, simple and accurate, and could be used for the quality control of this preparation.
出处
《浙江中医药大学学报》
CAS
2009年第3期422-423,共2页
Journal of Zhejiang Chinese Medical University
