摘要
目的探讨反义c-myc寡核苷酸对人骨肉瘤MG-63细胞增殖的影响及机制。方法采用反义核酸技术设计反义c-myc寡核苷酸片段,转染入人骨肉瘤MG-63细胞,MTT法及流式细胞仪法检测瘤细胞体外增殖、细胞周期及c-myc基因蛋白表达情况。结果MTT法示人骨肉瘤MG-63细胞的增殖受抑,10.0μmol/L、作用48 h效果最明显;流式细胞仪示反义c-myc寡核苷酸主要阻止人骨肉瘤MG-63细胞进入S期,c-myc基因蛋白表达受抑。结论反义c-myc寡核苷酸可抑制人骨肉瘤MG-63细胞的增殖;其机制为在基因水平干扰c-myc基因蛋白的表达。推测用反义策略抑制癌基因表达,或用基因敲除、基因替换的方法去除癌基因,可达到基因治疗的目的。
Objective To investigate the effect of the c-myc antisense oligonucleotide on the proliferation of osteosarcoma cells（MG-63） and its mechanism. Methods The c-myc antisense oligonucleotide fragment was designed by antisense oligonucleotide technique, and then transfected into human osteosarcoma MG-63 cell, the proliferation, cycle and c- myc protein expression of the cells were measured by MTr method and FCM method. Results The proliferation of human osteosarcoma MG-63 cell were inhibited by the c-myc antisense oligonucleotide, and the most notable effect was obtained when treated by the dosage of 10.0 μmol/L for forty-eight hours. The cycle of MG-63 cell into the S phase and the expression of c-myc protein were inhibited. Conclusions c-myc antisense oligonucleotide is able to inhibit the proliferation of human osteosareoma MG-63 cell, possible mechanism is interfere the expression of c-myc protein . Gene therapy can be used by inhibiting the expression of oncogene, or removing the oncogene by gene knock-out and gene replacement.
出处
《山东医药》
CAS
北大核心
2009年第19期1-3,共3页
Shandong Medical Journal
基金
贵州省教育厅自然科学研究基金资助项目(F2004-5)
关键词
寡核苷酸
反义
骨肉瘤
oligonucleotide, antisense
osteosarcoma
