摘要
以胚苗顶芽为外植体,开展湿地松离体培养初代培养基、增殖培养基的优化及组培快繁优良基因型的筛选试验。结果表明:最佳初代培养基为B5+6-BA 2.0 mg.L-1;年增殖率最大的增殖培养基为B5+6-BA 2.0mg.L-1+IBA 0.1 mg.L-1;试验从湿地松普通家系中筛选出增殖能力强的优良基因型132个,筛选出抗病家系优良基因型2个。
Using the sprouts of slash pine as explants, medium formulation for the first generation and multiplication was optimized. The results showed that the optimum medium for the first generation was B5 ＋ 6 - BA 2.0 mg · L^-1 , and B5＋6 - BA 2.0 mg·L^-1 ＋IBA 0.1 mg ·L^-1 for multiplication. The elite genotypes selected for the purposes included 132 from the non-antidisease and 2 from the antidisease families of the slash pine.
出处
《福建农业学报》
CAS
2009年第2期167-170,共4页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目(2007I0007)
关键词
湿地松
组培
器官发生
植株再生
优良基因型
Slash pine
tissue culture
organogenesis
plantlet regeneration
elite genotype
