摘要
目的观察核因子-κB（NF-κB）抑制剂吡咯烷二硫基甲酸酯（PDTC）对糖尿病大鼠肾组织细胞间黏附分子-1（ICAM-1）和血管内皮生长因子（VEGF）表达的影响。方法将30只雄性Wistar大鼠随机分成正常对照组（NC组）、糖尿病模型组（DM组）和PDTC干预组（DP组），每组10只。用链脲佐菌素腹腔注射诱导糖尿病大鼠模型。第8周末，收集24h尿液，检测各组大鼠尿微量白蛋白，处死大鼠后收集肾组织，使用免疫组织化学方法测定肾组织NF-κB、ICAM-1和VEGF的蛋白表达。结果①与NC组相比，DM组尿微量白蛋白升高，差异有统计学意义（P〈0．05）；与DM组相比，DP组尿微量白蛋白降低，差异有统计学意义（P〈0．05）。②与NC组相比，DM组肾组织NF-κB、ICAM1和VEGF蛋白表达升高，差异均有统计学意义（P〈0．05）；与DM组相比，DP组肾组织NF-κB、ICAM-1和VEGF蛋白表达降低，差异均有统计学意义（P〈0．05）。结论肾组织局部表达的ICAM-1和VEGF可能通过NF-κB途径参与糖尿病肾脏病的发生发展。
Objective To observe the expression of intercellular adhesion molecule-1 （ICAM-1） and vascular endothelial growth factor （VEGF） in the renal tissues of diabetic rats treated with the inhibitor of nuclear factor-kappaB （NF-κB） PDTC. Methods Thirty healthy male Wistar rats were randomly divided into 3 groups： normal control group （NC group）, diabetic model group （DM group） and diabetic rats with treatment of PDTC group （DP group） （each group having 10 rats）. Diabetic rats were induced by streptozotocin. At the 8th week, 24h urine was collected for UAER. The rats were killed, and renal tissues were isolated for the detection of the expression of ICAM-1, VEGF and NF-κB by using immunhistochemistry staining. Results （1）As compared with NC group, the urinary albumin was statistically significantly increased in DM group （P〈0. 05）; As compared with DM group, the urinary albumin was significantly reduced in DP group （P〈0. 05）. （2）The protein expression levels of NF-κB, ICAM-1 and VEGF in the renal tissues were significantly higher in NC group than in DM group DM （P〈0. 05）. The protein expression levels of NF-κB, ICAM-1 and VEGF in the renal tissues were significantly lower in DP group than in DM group （P〈0. 05）. Conclusion ICAM1 and VEGF produced locally in the renal tissues may be involved in the occurrence and progression of diabetic kidney disease, and inhibition of the NF-κB expression can reduce the expression of ICAM-1 and VEGF.
出处
《临床肾脏病杂志》
2009年第5期226-228,共3页
Journal Of Clinical Nephrology
关键词
糖尿病肾脏病
血管内皮生长因子
核因子-ΚB
动物
实验
Diabetic kidney disease
Vascular endothelial growth factor
Nuclear factor-kappa B
Animals, laboratory
