摘要
目的观察参芎注射液对肾缺血再灌注损伤大鼠肾组织核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性的影响，探讨其肾保护作用机制。方法将24只SD大鼠随机分为假手术对照组、缺血再灌注组、参芎预处理组，每组8只。免疫组织化学法检测各组大鼠肾组织NF-κB蛋白表达，酶联免疫吸附法检测肾组织TNF-α含量，用MDA和SOD试剂盒分别检测肾组织MDA含量和SOD活性。结果①与假手术对照组相比，缺血再灌注组大鼠肾组织NF-κB蛋白表达、TNF-α和MDA含量明显升高，差异均有统计学意义（P〈0．01）；而SOD的活性明显降低，差异有统计学意义（P〈0．01）。②与缺血再灌注组相比，参芎预处理组大鼠肾组织NF-κB蛋白表达、TNF-α和MDA含量降低，差异均有统计学意义（P〈0．05或P〈0．01）；而SOD的活性明显升高，差异有统计学意义（P〈0．01）。结论参芎注射液对肾缺血再灌注损伤有一定的保护作用，其机制可能与抗自由基氧化损伤以及抑制炎性细胞因子NF-κB和TNF-α的表达有关。
Objective To study the effect of shenxiong injection on renal expression of nuclear factor-kappaB （NF-κB）. tumor necrosis factor-α（TNF-α）, malondialdehyde （MDA） and superoxide dismutase （SOD） . Methods Twenty four SD rats were randomly divided into three group,sham operation group.ischemia-reperfusion group and shenxiong pretreatment group. The protein expression of NF-κB in renal tissue was detected by immunohistochemistry staining, TNF-α expression in renal tissue was analyzed by enzyme-linked immunosorbent assay method, MDA and SOD kits were used to detect MDA content and SOD activity in the renal tissue. Results （1）Compared with sham operation group, the protein expression of NF--κB, TNF-α and MDA content of ischemia-reperfusion group significantly increased, （P〈0.01） ; while SOD activity significantly decreased, （P〈0. 01 ）. （2）Compared with ischemia-reperfusion group, the NF-κB expression. TNF-α and MDA content in shenxiong pretreatment group decreased （P〈0. 05 or P〈0. 01） ; however, SOD activity significantly increased （P 〈0. 01）. Conelttsion Shenxiong iniection has a certain protective effect on renal ischemimreperfusion injury and the mechanism may be related with throughing against oxygen free radicals damage, restraining the expression of NF-κB and TNF-α.
出处
《临床肾脏病杂志》
2009年第5期229-231,共3页
Journal Of Clinical Nephrology
基金
湖北省科技攻关计划项目（编号：2007AA3018271）
