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布地奈德对A549细胞胸腺基质淋巴细胞生成素表达作用的研究 被引量:8

Effect of budesonide on the expression of thymic stromal lymphopoietin in A549 cells
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摘要 目的:观察细胞因子刺激气道上皮细胞胸腺基质淋巴细胞生成素(TSLP)表达是否涉及核因子κB(NF-κB),并探讨糖皮质激素布地奈德对气道上皮细胞TSLP表达和NF-κB核转位的影响。方法:A549细胞与细胞因子白介素1β(IL-1β)、白介素4(IL-4)和布地奈德共同孵育,以不加任何细胞因子或布地奈德培养的A549细胞为对照组,采用RT-PCR方法测定TSLP mRNA表达,细胞免疫荧光方法检测TSLP和NF-κB的表达情况。结果:与对照组比较,IL-1β(10ng/ml)及IL-4(10ng/ml)显著刺激A549细胞TSLP mRNA表达,且NF-κB(p65)核转位增加(均P<0.05)。布地奈德干预后TSLP mRNA的表达和NF-κB(p65)的核转位显著减少(P<0.05)。结论:细胞因子促进气道上皮细胞诱导性表达TSLP与NF-κB激活有关,抑制TSLP表达和NF-κB激活可能是布地奈德治疗哮喘的重要机制。 Objective: To investigate whether nuclear factor κB (NF-κB) is involved in the cytokine-induced expression of thymic stromal lymphopoietin (TSLP) and to explore the effect of budesonide on them. Methods: A549 cells were cultured with cytokines, interleukin1β (IL-1β) and IL-4, and budesonide (0.5 mg/ml). The control group: A549 cells were maintained without cytokines and drugs. The expressions of TSLP mRNA were detected by RT-PCR and the nuclear translocations of NF-κB (p65) were detected by immunofluorescence assays. Results: Compared with the control group, the expression ofTSLP mRNA and nuclear p65 were significantly increased in A549 cells stimulated with IL-1β, IL-4 or both of them (all P〈0.05), whereas that were greatly abolished in A549 cells pretreated with budesonide (P〈0.05). Conclusions: Cytokines enhance the inducible expression of TSLP in bronchial epithelial cells associated with NF-κB activation. Inhibition of NF-κB activation and TSLP expression might be an essential therapeutic mechanism of budesonide for asthma therapy.
出处 《现代生物医学进展》 CAS 2009年第10期1820-1823,F0002,共5页 Progress in Modern Biomedicine
基金 国家自然科学基金资助项目(30400191 30570797) 江苏省"六大人才高峰"项目(06B035) 江苏省医学重点人才项目(RC2007043)
关键词 胸腺基质淋巴细胞生成素 A549 布地奈德 NF—κB Thymic stromal lymphopoietin A549 Budesonide NF-κB
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