摘要
以龙眼特优质品种‘凤梨朵’为母本,大果型主栽品种‘大乌圆’为父本,杂交创建了‘凤梨朵’ב大乌圆’F1群体。从该杂种群体中随机选用94个单株作为作图群体,连同两个亲本品种,进行了RAPD、ISSR、SRAP和AFLP分子标记分析,并运用JoinMap3.0进行连锁分析,分别构建了‘凤梨朵’和‘大乌圆’的分子遗传图谱,其中,‘凤梨朵’的遗传图谱为21个连锁群,包含183个标记位点,覆盖总图距965.1cM,位点间平均遗传距离为5.84cM;‘大乌圆’的遗传图谱为22个连锁群,包含251个标记位点,覆盖总图距1064.8cM,位点间平均遗传距离为4.65cM。这是龙眼上首次报道的高密度分子遗传图谱,为后续的基因定位及辅助选择奠定了良好的基础。
A big F1 population of ＇ Fengliduo x Dawuyuan＇ were created by crossing between a high quality cultivar ＇ Fengliduo＇ and a large fruit size main cuhivar ＇ Dawuyuan＇. Among the hybrid population, 94 F1 individuals were randomly chosen and used as the mapping population. Based on the mapping population, a molecular genetic linkage map of longan was constructed with RAPD, ISSR, SRAP and AFLP molecular markers. According to the linkage analysis by JoinMap3.0, the molecular linkage map of ＇ Fengliduo＇ fell into 21 linkage groups, which contained 183 markers, with an average interval of 5.84 cM and covered a total distance of 965. 1 cM ; While the molecular linkage map of ＇ Dawuyuan＇ fell into 22 linkage groups, which contained 251 markers, with an average interval of 4. 65 cM and covered a total distance of 1 064. 8 cM. This was the first report about high-density molecular genetic map for longan and would lay a solid foundation for gene location and marker assited selection in longan breeding.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期655-662,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30771498)
中国博士后科学基金项目(20080440763)
广东省科技计划项目(2KM03001N)
关键词
龙眼
分子标记
遗传图谱
longan
molecular marker
genetic map
