摘要
参照水体中浮游植物叶绿素a的萃取测定方法,运用丙酮、乙醇、二甲基甲酰胺(DMF)、二甲基亚砜(DM-SO)4种有机溶剂对不同土质不同发育阶段自然和人工生物结皮中叶绿素a的萃取效率进行了比较研究。结果发现,不同有机溶剂从生物结皮中萃取叶绿素a的效率存在极显著差异(P<0.001),而乙醇法的离散度最低;取样量对萃取效率总体上没有显著影响(P>0.05),但在具体的测定中与萃取效率之间存在负相关。4种方法中,DMSO法对藻类占优势的结皮萃取率最高,DMF法对自然结皮的萃取率仅次于DMSO法,在人工结皮中最低;乙醇法和丙酮法的萃取效率普遍较低,而且与结皮属性之间没有明显规律。
Based on the extraction and measurement of phytoplanktonic Chl-a in water, this study compared contents of Chl-a extracted by acetone, ethanol, N, N-Dimethylformamide （DMF） and Dimethyl Sulphoxide （DMSO） in natural and man-made biological crusts that were in different soil texture and stages of development. The results showed that there was significant statistical difference in content of Chl-a extracted by different organic solvents （P〈0. 001）, while scatteration was the least in ethanol method. Although the sample content hadn＇t prominent effects on content of Chl-a extraction as a whole （P〉0. 05）, there was a negative correlation between content of Chl-a extraction and sample content. Comparisons displayed that in the algae dominative crusts, content of Chl-a extraction was the highest in DMSO method, while lower in DMF than DMSO method in natural crusts, but the lowest in the man-made crusts. Generally, the extraction rate was lower in acetone and ethanol method, which also hadn＇t evident relation to the crusts＇ attributes.
出处
《中国沙漠》
CSCD
北大核心
2009年第3期524-528,共5页
Journal of Desert Research
基金
国家自然科学基金项目(30770395)
中国科学院知识创新领域前沿项目
武汉市科技局与内蒙古发改委重大科技产业化专项资助
关键词
有机溶剂
萃取
生物结皮
叶绿素A
organic solvent
extraction
biological crust
Chl-a
