摘要
本研究旨在分析HSL基因多态性与地方猪品种瘦肉率之间的相关性。采用RFLP方法,以二元杂交猪(长白猪×大约克猪)为对照,对贵州地方猪品种糯谷猪和萝卜猪的HSL基因进行多态性分析。结果表明,贵州地方猪品种HSL基因外显子1中存在BsaH I酶切片段长度多态性,从3个猪群体中都检测到AA、AB和BB基因型,克隆测序后证实,A等位基因于第442 bp处发生了单碱基突变(G→A),导致氨基酸Val→Ile替换。地方猪品种以A等位基因占优势,杂交猪以B等位基因占优势。将猪胴体性状与HSL基因型之间进行关联分析,结果显示大部分年龄段的猪品种中,BB、AB和AA 3种基因型的瘦肉率和眼肌面积均表现出从高到低的变化趋势,而背膘厚则完全相反。结果提示,HSL外显子1的B等位基因具有增加胴体瘦肉率和眼肌面积、降低背膘厚的遗传效应,可作为贵州地方猪品种瘦肉率的分子标记。
The aim of the present study was to investigate the association of the polymorphism of HSL gene with carcass traits in native pig breeds in Guizhou province. Two native pig breeds in Guizhou province, Nuogu pig （n=30） and Luobo pig （n=50）, were chosen with the crossbreed of Large WhiteX Landrace （n=30） as control. The polymorphism of HSL exon 1 was detected by restriction fragment length polymorphism （RFLP） method. Three genotypes, AA, AB and BB, were detected from the HSL gene in three pig populations. It showed that A allele was dominant in the two native pig breeds with 60% in Nuogu pig, 89.5% in Luobo pig and the B allele was detected mainly from the European crossbreed with 91.7%. Compared with B allele, a single nucleotide mutation （G/A） was confirmed in A allele at the 442 bp of the coding region in exon 1 of HSL gene which resulted in the amino acid change from valine to isoleucine. Moreover, the lean percentage, backfat thickness and loin eye area of individuals with BB genotype were significant superior than that with AA genotype （P〈0.01）. These results indicated that the B allele committed to improve the lean percentage and loin eye area, and decreased the backfat thickness.It suggested that HSL gene could be a molecular marker for the selection of lean percentage in the native pig breeds.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第5期627-632,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
贵州省优秀青年科技人才项目(黔科合人字(2005)0506号)
贵州省“十一·五”农业科技重大专项(黔科合NZ字2005-3002)
贵州大学研究生创新基金(省研农2006003)
