摘要
目的:利用基因芯片技术筛选不同浓度蔗糖条件下变形链球菌中差异表达的基因。方法:构建变形链球菌基因表达谱检测芯片,利用基因芯片技术筛选不同浓度蔗糖环境中变形链球菌差异表达的基因,以观察不同浓度蔗糖环境对变形链球菌基因表达的影响。结果:0.5%蔗糖环境相对于1.0%蔗糖环境,变形链球菌中有约40种基因表达发生显著变化,其中与变形链球菌毒力、致病性、信号系统、细菌表面成分、脂肪酸和磷脂代谢和DNA复制、重组、修复等相关的23种基因表达显著下调,而糖转运系统和能量代谢等相关的17种基因表达明显上调(P<0.05)。结论:不同浓度蔗糖环境对变形链球菌的调控是多因素、多基因共同作用的结果;基因芯片能有效地筛选不同环境中变形链球菌差异表达的基因。
Objective：To detect the differently-expressed genes of S. mutans cultured in 0.5% and 1% sucrose by microarray technique. Methods ： Gene chips were constructed and the gene expressions of S. mutans under different conditions were detected. Results： In 0.5% sucrose environment, compared with 1.0% sucrose environment, expressions of about 40 S. mutans genes were changed. 23 genes associated with virulence, pathogenesis, bacterial component metabolism, and so on, were down-regulated. While 17 genes related to sugar transport and energy metabolism were up-regulated. Conclusion： Regulation of S. mutans by environment factors is very complex and is a result of cooperations of multifactors and multigenes. Microarray technique is efficient to detect differently expressed genes in S. mutans.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2009年第3期335-339,共5页
Journal of Practical Stomatology
基金
国家自然科学基金资助项目(编号:30200310)
湛江市科技攻关项目(编号:2008C02001)
关键词
基因芯片
变形链球菌
基因表达
Microarray
Streptococcus mutans
Gene expression
