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海马内注射内皮素-1导致大鼠痫性发作和海马硬化的初步研究 被引量:8

Intrahippocampal injection of endothelin-1 induces seizures and hippocampal sclerosis in mature rats.
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摘要 目的观察大鼠海马内注射内皮素(endothelin,ET)-1是否导致大鼠痫性发作和海马硬化。方法立体定位在成年大鼠海马CA3区内分别注射1μL ET-1(200 pmol,15只)、海人酸(kainate,KA5 pmol,15只)或磷酸盐缓冲液(PBS,0.01 mol,8只),观察大鼠行为学、脑电及对侧海马病理学改变。结果海马注射PBS后大鼠未见痫性发作,脑电图呈10~15 Hz、150~200μV基本节律。注射ET-1或KA2 h内大鼠出现不同程度的痫性发作和脑电图异常改变(尖波或尖慢波),KA组3~5级发作率高于ET-1组(86.67%vs 16.67%,P<0.05)。部分ET-1和KA组大鼠在给药后2~3周可见癫痫样发作行为学改变。与PBS组比较,ET-1和KA组给药后48 h对侧海马各区Nissl染色细胞数明显减少,GFAP表达增强(P<0.05);给药后30 d,对侧CA3区和门齿区苔藓纤维出芽评分高于PBS组(P<0.05)。结论海马注射ET-1可以导致大鼠癫痫样行为改变和海马硬化。 Objective To investigate whether intrahippocampal injection of endothelin (ET)-1 can induce epileptic seizures and hippocampal sclerosis in mature rats. Methods ET-1 (200 pmol, 15 rats), kainate (KA, 5 pmol, 15 rats) or PBS (0.01 mol, 8 rats) was injected into the right dorsal hippocampus of Sprague-Dawley male rats in injection volume of 1 uL for each compound, respectively. All rats were then monitored for 2 h with a Nissl, glial fibrillary acidic protein (GFAP) and Nero-Timm staining was performed at 48 h and 30 d after the injection respectively. Results Animals receiving PBS did not have seizure and their electroencephalographic activities were 10 - 15 Hz, 150 - 200 μV. Both ET-1 and KA treated rats demonstrated different kinds of seizures accompanied with electrographic seizure activities (slow, or sharp-slow waves). The percentage of grade 3 - 5 seizure rate was significant higher in KA group than in ET-1 group (86. 67% vs 16. 67% ,P 〈0. 05). Seizures could be observed in some ET and KA treated rats even 2 - 3 weeks after the injection. Compared with PBS treated controls, KA and ET-1 treated rats exhibited less Nissl stained cells, more GFAP staining cells, and higher MFS score at the contralateral hippocampus following injection (P 〈 0. 05, respectively). Conclusions Intrahippocampal injection of ET-1 can induce epileptic seizure activities and hippocampal sclerosis in rats.
出处 《中国神经精神疾病杂志》 CAS CSCD 北大核心 2009年第5期276-279,共4页 Chinese Journal of Nervous and Mental Diseases
基金 广东省自然科学基金资助(编号:5006055)
关键词 内皮素-1 癫痫 海马硬化 海人酸 Endothelin-1 Epilepsy Hippocampal sclerosis Kainate
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参考文献15

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二级参考文献19

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