摘要
目的探讨具有清热利湿、祛风通络作用的中药制剂悉通颗粒治疗痛风性关节炎的作用机制。方法32只雌性Wistar大鼠随机分为空白对照组、模型组、秋水仙碱组(阳性对照)、悉通颗粒组,以尿酸钠溶液注射大鼠踝关节造成痛风性关节炎模型,ELISA法检测大鼠TNF-α水平,免疫组化法观察大鼠关节软组织TGF-β1及TGF-βR1的表达。结果悉通颗粒组大鼠关节软组织TNF-α水平降低,TGFβ1及TGFβR1表达增加,与模型组比较有显著差异。结论悉通颗粒治疗痛风性关节炎的机制可能为通过抑制TNF-α致炎因子,上调TGF-β1及TGF-βR1的适度表达。
Objective： To explore the therapeutic mechanism of Xitong Granule in treating gouty arthritis. Methods： 32 female Wistar rats were randomly divided into four groups： control, model, colchieine and Xitong Granule group. The monosodium urate was injected into ankle joint cavity for gouty arthritis model establishment. The level of TNF - α was measured by ELISA. The expression of TGFβ1 and TGFβR1 were examined by immunohistochemistryt. Results： The level of TNF - α in soft tissue surrounding ankle joint in Xitong Granule group was lower than those in model group, but the expression of TGFβ1 and TGFβR1 was significantly increased. Conclusion： The mechanisms of Xitong Granule treating gouty arthritis may be through inhibiting levels of TNF - α and up - regulating the expression of TGFβ1 and TGFβR1 of soft tissue surrounding ankle joint.
出处
《中国中医急症》
2009年第6期956-957,共2页
Journal of Emergency in Traditional Chinese Medicine
