摘要
应用逆转录－套式聚合酶链反应（ＲＴ－ｎＰＣＲ）法，对２６例散发性戊型肝炎（ＨＥ）患者发病后４～２８天收集的９１份粪便标本进行ＨＥＶＲＮＡ检测，并与同病期系列血清ＨＥＶＲＮＡ及ＡＬＴ水平作比较，以了解散发性ＨＥ患者的粪便排病毒规律。结果表明，患者粪便ＨＥＶＲＮＡ阳性率为４２．３％（１１／２６），与血清ＨＥＶＲＮＡ阳性率（５３．８％）相比无显著性差异（Ｐ＞０．０５）。粪便ＨＥＶＲＮＡ检出率在发病头５天内为７５％（３／４），随后即与血清ＡＬＴ水平一样出现明显下降，于发病后１６～２０天降至１９．２％（５／２６），最长一例持续阳性至发病后２３天。上述结果提示，ＨＥ患者的粪便排病毒主要发生在疾病的急性期早期，将其隔离期定为病后３周可能比较合理。
reverse transcriptionnested polymerase chain reaction(RTnPCR)assay was used for the detection of HEV RNA in 91 fecal specimens collected from 26 hospitalized patients with sporadic hepatitis E.The patterns of virus shedding in feces were compared with viremia of HEV,as well as the dynamics of serum ALT levels.The results revealed that 11 of 26(423%)patients showed HEV RNA in feces,compared with 533% in serum.The positive rate of HEV RNA in fecal specimens was 75%(3/4) within 5 days of illness,and declined dramatically with the course of the disease in consis tence with the levels of serum ALT.Only 5 of 26(192%) fecal specimens obtained from 16 to 20 days of illness were HEV RNA positive,1 of which persisted shedding up to the 23rd day after onset of the disease.It suggest that excretion of virus in stools of patients with hepatitis E happens during early acute phase of the disease,and the clinical isolation period of three weeks after onset of illness may be reliable.
出处
《中国公共卫生》
CAS
CSCD
北大核心
1998年第4期229-231,共3页
Chinese Journal of Public Health
基金
浙江省自然科学基金
浙江省教委及浙江省卫生厅共同资助
