摘要
采用2.4 U/mL Dispase酶消化和机械切割法分离毛囊隆突部,经胰酶(0.5 mg/mL胰酶+0.2 mg/mL EDTA)消化,从山羊耳部皮肤分离得到毛囊干细胞,并检测了不同浓度的IGF-1对山羊毛囊干细胞体外培养的作用。结果表明,IGF-1对毛囊干细胞体外培养作用很明显,并且最适IGF-1浓度是10 ng/mL。
Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA. The results showed that cells were cultured in different concentrations of IGF-1, IGF-1 had a good effect on the goat hair follicle stem cells in vitro and the 10 ng/mL was best concentration.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第5期127-129,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30200137)
国家“863”计划(2002AA216161)
教育部重大科技专项(03160)
关键词
毛囊干细胞
克隆形成率
IGF-1
体外培养
hair follicle stem cells
colony-forming efficiency
IGF-1
in vitro culture
