摘要
选用254份黄淮麦区小麦品种(系),利用BFMR1,DFMR2和微卫星xgwm261标记检测了矮秆基因Rht-B1b,Rht-D1b和Rht8的分布.结果表明,在254份材料中,含有Rht-B1b,Rht-D1b和Rht8基因的材料分别有84,171和178份,平均株高分别为80.7,78.5和80.7 cm.只含有Rht-B1b,Rht-D1b和Rht8基因的材料分别有15,36和31份,平均株高分别为83.8,80.1和86.2 cm.只含Rht-B1b和Rht-D1b基因有16份,平均株高为73.7cm,Rht-B1b和Rht-D1b基因具有累加效应,两个基因同时存在时株高降低幅度会更大.只含Rht-B1b和Rht8基因的有94份,只含Rht-D1b和Rht8基因的有28份,同时含有Rht-B1b,Rht-D1b和Rht8基因的有25份,同时不含这3个矮秆基因的有9份,说明黄淮麦区小麦品种(系)中绝大部分品种均含有不同种类的矮秆基因.微卫星WMS 261及基于PCR的2个STS标记可以分别用于对品种(系)中Rht8,Rht-B1b和Rht-D1b基因型的鉴定以及育种世代该基因型的筛选.
The distribution of Rht-B1b, Rht-D1 b and Rht8 for 254 wheat lines from Huang-huai wheat area was studied by means of molecular markers BFMR1, DFMR2 and Xgwm261. The results showed that 84, 171 and 178 lines were found to take Rht-B1b, Rht-D1b and Rht8 gene respectively, and that the corresponding average height was 80.7, 78.5 and 80.7 cm respectively. The number of lines only with Rht-B1b, Rht-D1b and Rht8 was 15, 36 and 31 respectively, and the corresponding average height was 83.8, 80. land 86.2 cm respectively. Sixteen lines only took the genes of Rht-B1b and Rht-D1b and their average height was 73.7 cm. Additive effect was found between Rht-B1b, and Rht-B1b, and when the two genes existed simultaneously in a variety, its plant height would be lower. Ninety-four lines only took the gene of Rht-B1b and Rht8, twenty-eight only took Rht-D1b and Rht8, twenty-five took Rht-B1b, Rht-D1b and Rht8, and nine didn＇t take any of the three dwarfing genes. Therefore, most germplasms from Huang-Huai wheat area take different dwarfing genes, and the three molecular markers are feasible to test the dwarfing genes and to be applied in assistant selection breeding.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2009年第2期118-125,共8页
Journal of Henan Agricultural University
基金
国家“863”高技术研究与发展计划项目(2006AA100102)
关键词
小麦
矮秆基因
分子标记
wheat
dwarfing gene
molecular marker
