摘要
隐球酵母(Cryptococcus sp.)C331菌株是从灵武长枣果实表面分离筛选得到的一种拮抗酵母菌。接种优势致病菌黑根霉(Rhizopus sp.)后,研究拮抗菌和致病菌诱导果实自身抗真菌性能的影响。通过离体抑菌法和活体抑菌法,以及PPO、POD和SOD酶活测定,用EXCEL和SAS软件分析数据。结果表明,隐球酵母C331菌株能有效抑制长枣采后病害的发病率和病斑直径,其拮抗效果与拮抗菌的使用浓度成正比;拮抗菌能显著地提高PPO和SOD活性,降低POD活性。研究证明隐球酵母C331菌株能显著抑制长枣采后病害,提高果实自身抗真菌性能。
Crptococcus C331, isolated from Lingwu long jujube fruits, was effective antagonist yeast for control of postharvest diseases. The induction of antifungal activity by infection of C331 and Rhizopus sp. was investigated. The changes in PPO, POD and SOD were measured in vitro and in vivo. The results showed that C331 could signifi-cantly reduce disease incidences and lesion diameters, and there was a significant positive correlation between the concentrations of C331 and its antagonistic activity; C331 could increase the activities of phenoloxidase （PPO） and superoxide dimutese （SOD） SOD, and decrease the activities of peroxidase （POD）. Cryptococcus C331 could control the Dost-harvest diseases and increase the antifungal activitv of long jujubes.
出处
《植物保护》
CAS
CSCD
北大核心
2009年第3期77-81,共5页
Plant Protection
基金
宁夏科技厅重点攻关课题(编号2006FM01)
