摘要
根据已报道的菊花B病毒(CVB)和番茄不孕病毒(TAV)外壳蛋白基因序列合成两条寡核苷酸引物,用RT-PCR的方法对这两种病毒的外壳蛋白基因进行了扩增,得到与预期大小相符的特异扩增条带。将其克隆到pGEM-T中,经序列分析表明所克隆的是CVB和TAV的CP基因,与已知的序列相比,CVB的同源性分别为82%、85%,而TAV的同源性为98%。利用两重RT-PCR成功同步检测这两种病毒,从而建立了一种能同时检测两种病毒的快速、简便、灵敏的分子检测方法。
A pair of primers were designed and synthesized based on the reported nucleotide sequences of the coat protein （CP） gene of Chrysanthemum virus B （CVB） and Tomatoaspermy virus （TAV）. The CP genes of the CVB and TAV were cloned by reverse transcription-polymerase chain reaction （RT-PCR）, and the expected sizes of the viruses were amplified. The products were cloned into pGEM-T-Easy vector and sequenced. The identity of CVB and TAV with the nucleotide sequences of the reported genes are 82% and 98%, respectively. The two viruses could be detected successfully by double RT-PCR.
出处
《植物保护》
CAS
CSCD
北大核心
2009年第3期89-90,共2页
Plant Protection
基金
北京市科委特色花卉重大项目(D0606003040191)
北京市农林科学院青年基金(2007020213)
北京市农委项目(20070136)
