摘要
为了缓解酒糟离心液对环境的污染,达到废物资源化的目的,以餐厨垃圾酒糟离心液和麸皮为基础培养基,经黑曲霉发酵制取糖化酶.采用Plackett-Burman试验优化产糖化酶培养基,并对比自制糖化酶和工业酶制剂应用于餐厨垃圾乙醇发酵的能力.结果表明硫酸铵、氯化铵、酵母粉、豆饼、玉米粉、硫酸镁、磷酸氢二钾、氯化钙等8个因素中氯化钙为影响发酵的显著性因素.当氯化钙添加量为0.2%时,所产糖化酶的酶活最高,达3404.44 U/ml.自制糖化酶与工业酶制剂应用于餐厨垃圾酒精发酵时的酒精产量仅差6%.表明自制糖化酶可替代工业糖化酶运用于餐厨垃圾酒精发酵工艺中,这不仅可降低糖化酶的生产成本和餐厨垃圾酒精发酵成本,同时也可减少酒糟离心液对环境的污染.
In order to avoid pollution caused by distillery waste from ethanol production by kitchen garbage, optimum conditions for glucoamylase production from such waste were determined in this study. Plackett- Burman design was utilized to screen the most influential factors of the fermentation medium. Co optimum ingredients as well as conditions were studied. Then the produced enzyme was adopted production to evaluate its feasibility compared with industrial product. The test on eight added rresponding for ethanol ingredients, namely （NH4）2SO4, NH4Cl, yeast extract, soybean powder, corn powder, MgSO4, KH2PO4 and CaCl2, demonstrated that only CaCl2 was the crucial factor during fermentation. The maximum glucoamylase 3404.44 U/ml was obtained at the addition 0. 2% of CaCl2. Ethanol production by enzymes produced from distillery waste was only about 2 g/L less than that by industrial product. This proves that the glucoamylase produced in this study could be used to take the place of industrial product. This method saves the cost of enzymes and decreases the pollution caused by distillery waste from ethanol production.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2009年第4期58-61,共4页
Journal of Harbin Institute of Technology
基金
“十一五”国家科技支撑计划重大资助项目(2006BAJ04A06)
