摘要
目的 研究CYP1A1多态性与焦炉工尿中1-羟基芘水平的关系。方法 按照苯并pa]芘（B[a]P）接触水平将223名焦炉工分为炉顶组（76名）、炉侧组（82名）和炉底组（65名），以119名无职业性接触多环芳烃的工人为对照组，采用聚合酶链反应-限制性片段长度多态性分析（PCR—RFLP）CYP1A1基因MspI位点多态性、等位基因特异性扩增方法检测CYP1A1基因1462V位点的多态性。结果 炉顶组、炉侧组和炉底组焦炉工尿中1-羟基芘水平分别为（3．77±0．64）、（3．57±0．49）、（3．26±0．80）μmol／mol Cr，明显高于对照组，差异有统计学意义（P〈0．01）。各组CYP1A1 MspI位点各基因型的尿中1-羟基芘水平差异均无统计学意义（P〉0．05）。炉侧组和炉顶组CYP1A1 1462V位点的各基因型尿中1-羟基芘水平均为Ⅱe／Ⅱe〈Ⅱe／Val〈Val，Val，差异有统计学意义（P〈0．01）。多元直线回归分析表明，尿中1-羟基芘水平主要受B[a]P接触水平和CYP1A1 1462V位点基因型的影响；logistic回归分析表明，炉顶组、炉侧组和炉底组尿中1-羟基芘增高的OR值分别为对照组的24．926、6．729和4．226倍，CYP1A1 MspI位点m2／m2和ml／m2基因型尿中1-羟基芘增高的OR值分别为ml／ml基因型的4．031和1．794倍，Val／Val和Ile／Val基因型尿1-羟基芘增高的OR值分别为Ile／ile基因型的5．524和3．811倍。结论 焦炉工尿1-羟基芘水平与工作环境中苯并[a]芘暴露趋势一致，CYP1A1 MspI位点和1462V位点基因多态性影响焦炉工尿中1-羟基芘的水平。
Objective To study the associations of CYP1A1 gene polymorphisms with levels of urinary 1-hydroxypyrene among coke oven workers. Methods 223 male workers from a coke plant （76, 82 and 65 workers in oven top group ,oven-side group and oven-bottom group respectively） and 119 controls without occupational polycyclic aromatic hydrocarbons exposure were selected. The MspI gene polymorphism in CYP1A1 3＇flanking region and the genotypes at I462V site in exon 7 of CYP1A1 were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and allele specific amplification （ASA）. Results The urinary 1-hydroxypyrene of coke oven workers in oven-top,oven-side and oven-bottom（3.77±0.64,3.57±0.49,3.26±0.80 μmol/mol Cr） were significantly higher than controls（2.80±1.02 μmol/mol Cr）（P〈0.01）. The urinary 1-hydroxypyrene was not significantly different among MspI genotypes in CYP1A1 3 ＇flanking region （P〉0.05）. In oven-top group and oven-side group,the subjects with Val/ Val genotype in exon 7 of CYP1A1 had significantly higher urinary 1-hydroxypyrene levels than those with Ile/Val or Ile/Ile genotype,and urinary 1-hydroxypyrene of Ile-Val genotype were also significantly higher than Ile/Ile genotype （P〈0.01）. Multivariate logistic regression analysis showed that the coke oven workers （OR in oven top group,oven-side group and oven-bottom group was 24.926,4.226 and 6.729 respectively） and subjects with m2/m2 genotype in CYP1A1 3＇flanking region （OR=4.031） or with Val/Val or Ile/Val genotype in exon 7 of CYPIA1 （OR were 5.524 and 3.811 ） had elevated urinary 1-hydroxypyrene （greater than 95 percentile of control group,3.876 μmol/mol Cr）. Conclusion BAP concentration of work environment contributes to the elevated urinary 1-hydroxypyrene levels, and the exposed BAP levels were regnlated by the CYP1A1 MspI and I462V genotypes. Genetic polymorphism of CYP1A1 gene could be a susceptible biomarker in coke oven workers which was involved in the individual susceptibility on metabolism of PAHs.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2009年第5期270-275,共6页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金资助项目（30471437）
