摘要
目的：说明丹参酮ⅡＡ对血管合成血管紧张素Ⅱ及醛固酮的作用。方法：用大鼠肠系膜动脉离体灌注、高效液相色谱法和放射免疫法检测肠系膜动脉中合成的血管紧张素Ⅱ及醛固酮。用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）及ＳｏｕｔｈｅｒｎＢｌｏｔ法检测血管中醛固酮合酶基因ＣＹＰ１１Ｂ２信使核糖核酸（ｍＲＮＡ）的表达情况。结果：丹参酮ⅡＡ抑制血管合成醛固酮，但不能抑制血管合成血管紧张素Ⅱ。丹参酮ⅡＡ抑制血管中醛固酮合酶基因ＣＹＰ１１Ｂ２ｍＲＮＡ的表达。结论：丹参酮ⅡＡ治疗心血管疾病的机制可能与其抑制血管合成醛固酮有关。
Objective:To find the pharmacological effects of tanshinone ⅡA on angiotensin Ⅱ and aldosterone production in blood vessels. Methods:Using ex vivo rat mesenteric artery perfusion,HPLC,and RIA to measure the amount of angiotensin Ⅱ and aldosterone produced in vascular tissue,and by RT PCR and Southern Blot to detect the expression of aldosterone synthase gene—CYP11B2 mRNA in aorta. Results:The production of aldosterone but not of angiotensin in mesenteric artery was inhibited ( p <0.05) and the CYP11B2 mRNA expression in aorta was down regulated by tanshinone ⅡA. Conclusion:The inhibition of vascular aldosterone by tanshinone ⅡA might contribute to its therapeutic effect on cardiovascular diseases.
出处
《中国循环杂志》
CSCD
北大核心
1998年第3期175-177,共3页
Chinese Circulation Journal
关键词
丹参酮ⅡA
醛固酮
醛固酮合酶基因
MRNA
中药
Tanshinone ⅡA
Aldosterone
Angiotensin Ⅱ
CYP11B2 mRNA
Ex vivo mesenteric artery perfusion
