摘要
目的:体外诱导骨髓间充质干细胞(MSC)向软骨细胞定向分化,并对分化后的细胞进行鉴定方法:由健康兔子骨髓中分离出MSC,取第4代细胞进行实验。鉴定后,将细胞在地塞米松,bFGF,维生素C及TGF-B等诱导下分化后,通过RT-PCR检测Ⅱ型胶原(ColⅡ)的表达结果:诱导分化后的MSC可表ColⅡ的mRNA。结论:MSC在地塞米松,bFGF,维生素C及TGF-B等诱导后,可分化为软骨细胞。
Objects： To induce bone marrow mesenchymal stem celts or bone marrow stroma celts （MSCs） to differentiate directionally into chondrocytes in vitro and then identify the differentiated cells. Methods： MSCs were obtained from bone marrows of healthy rabbit and then the cultured MSCs at the 4 passage were induced by TGF, dexamethasone （Dex）, bFGF and Vitamin C （Vit C） . Later, the expression of Col Ⅱ in the induced MSCs cell were detected by RT-PCR. Results： The induced cells exhibited a chondrocyte like morphology. PCR showed that Col Ⅱ was only expressed in the induced cells. Conclusion： MSCs can differentiate into chondrocytes under the induction of TGF, dexamethasone （ Dex）, bFGF and Vitamin C （ Vit C）.
出处
《沈阳医学院学报》
2009年第2期79-81,共3页
Journal of Shenyang Medical College
基金
辽宁省科技厅资助项目(No.2005225017)
关键词
间充质干细胞
软骨细胞
分化
mesenchymal stem cell
chondrocyte
differentiation
