摘要
目的:建立HPLC测定白头翁药材中白头翁皂苷B4含量的方法,并比较不同产地、不同生长年份对白头翁皂苷B4含量的影响,以评价白头翁药材质量。方法:HPLC流动相:乙晴∶水(28∶72);流速:1.0 mL/m in;分离柱采用ZORBAX SB-C18(4.6 mm×150 mm,5μm),反相填料;柱温:室温;检测波长:205 nm。结果:皂苷B4进样量在0.312 5-10μg范围内与吸收峰面积呈良好的线性关系(r=0.999 5,n=6),平均加样回收率为99.74%,RSD=3.28%(n=3)。结论:该方法简便、准确、重现性好,可用于白头翁皂苷B4的含量测定;白头翁中皂苷B4含量以安徽凤阳产药材较高,可作道地药材。
Objective：To establish a HPLC method to determine the contenis of saponins B4 in Pasque flower and study the effects of different predocing area and period of growth on the conients of saponins B4.Methods：With HCN and H2O（28：72）as flow phase and 1mL/min flow rate,a opposite phase C18 column（ZORBAX SB-C18,4.6mm×150mm,5μm）was used to separate the targel component under room temperature,then was detected at 205nm.Results：The absorbance area abowed significantly linear relation（r=0.9995,n=6）to the quantity of Saponon B4 in the range from 0.3125μg to 10μg.The tested sample recovery was 99.74% and the relative SD was 3.28%（n=3）.Conclusion：The HPLC method is quite feasible,precise and reproducible ,so it is suitable for determining saponin B4 cotent in medicinal material Pasque flower.The masterial in Fengyang of Anhui Province gives a higher production of saponin B4.
出处
《中兽医医药杂志》
2009年第3期7-9,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
安徽省教育厅优秀人才基金项目(2006JQ1196)
安徽省安徽科技学院引进人才专项(ZRC200684)
