摘要
目的探讨RNA干扰沉默乙酰肝素酶基因对卵巢癌微血管内皮细胞的影响。方法实验组为乙酰肝素酶siRNA转染组(转染组),实验对照为正常组和siRNA空白转染对照组(空白转染组),观察时间为转染后2、4、6、8d。观察血管内皮细胞乙酰肝素酶siRNA转染效率,用MTT法检测细胞活力,流式细胞仪检测细胞周期变化,免疫荧光、RT-PCR法及WB法检测内皮细胞乙酰肝素酶表达。结果转染组细胞生长受抑制,增殖指数显著下降,乙酰肝素酶mRNA水平和蛋白水平显著下降,细胞凋亡率显著增加。结论RNAi可有效沉默卵巢癌微血管内皮细胞乙酰肝素酶表达,提示RNAi可为卵巢癌治疗提供新的途径。
Objective To explore the effect of RNA interference on the heparanase expression of blood vessel endothelium cell of ovarian cancer. Methods In this study,the experiment group was the heparanase RNA interference group. The control groups were the normal cell group and the heparanase RNA interference blank group. The observing time were 2d,4d,6d and 8d after RNA interference. The interference efficiency was observed. MTT assay, flow cytometry,immunofluorescence, WB and RT-PCR were used to detect the cell changes of these three groups. Results In the experiment group,heparanase mRNA level and protein significantly de＆eased,tl^e proliferation index significantly decreased and apoptosis index significantly increased. Conclusion RNA interference can inhibit heparanase expression and cell proliferation efficiently. RNAi is one way for the treatment of tumor.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第11期1367-1369,F0003,共4页
Chongqing medicine
