摘要
以铁皮石斛Dendrobium officinale的茎尖为外植体,在特定条件下诱导产生拟原球茎,进一步培养形成幼苗。结果表明:原球茎最优诱导培养基为1/2MS(Murashige and Skoog)+0.5mg·L-16-苄基嘌呤(6-BA)+0.05mg·L-1萘乙酸(NAA)+0.1mg·L-1激动素(KT)+1.0g·L-1水解乳蛋白(LH)。复合添加物的试验表明,土豆汁对拟原球茎(PLBs)的增殖有很好的促进作用。对铁皮石斛拟原球茎的解剖学研究表明:拟原球茎是由位于顶端分生组织下的薄壁细胞脱分化形成的胚性细胞发育而来。外植体接种后20d左右外植体基部开始膨大,40d左右出现肉眼可见的乳白色突起,这些突起逐渐增大,成为圆形至卵圆形的乳白色拟原球茎。在条件适宜的拟原球茎增殖培养基上继续培养,拟原球茎边缘的薄壁细胞继续脱分化,进一步发育形成球形的细胞团,可继续形成突起,使拟原球茎不断增殖。
To determine the most effective medium for protocorm-like body （PLB） induction and growth, shoot tips of Dendrobium officinale were cultured on media. Then, several compound addenda were added to the media to determine the best treatment for PLB multiplication, and this was followed by anatomical observation of PLB formation on D. officinale. Results showed that the best medium for PLB induction and embling regeneration was 1/2MS ＋ 0.5 mg·L^-1 6-BA ＋ 0.05 mg·L^-1 NAA ＋ 0.1 mg·L^-1 KT ＋ 1.0 g·L^-1 LH with potato juice. The anatomical observation showed that the PLBs developed from the tissue behind the apical meristem, which could dedifferentiate into embryogenetic cells. The process was as follows： creamy global structures were visible after 40 d, and these gradually increased. Cells on the edge of the PLBs continued to dedifferentiate forming a global colony. These global structures grew into bumps when the PLBs multiplied.
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2009年第3期444-448,共5页
Journal of Zhejiang Forestry College
关键词
植物学
铁皮石斛
拟原球茎
发生
组织培养
botany
Dendrobium officinale
protocorm-like body（PLB）
development
tissue culture
