摘要
目的:研究夏枯草对人结肠癌SW480细胞FasL mRNA表达及对其侵袭能力的影响,为中药抗肿瘤提供实验依据。方法:根据MTT法得到夏枯草对SW480细胞的半数有效抑制浓度(IC50),确定药物作用浓度。SW480细胞分别经夏枯草不同浓度(0.5IC50、IC50)作用后,应用逆转录-聚合酶链反应(RT-PCR)法检测夏枯草作用前后人结肠癌SW480细胞FasL mRNA的变化;应用Transwell细胞侵袭试验检测夏枯草对SW480细胞侵袭能力的影响。结果:夏枯草处理后较处理前SW480细胞FasL mRNA表达水平均明显高于对照组(P<0.01);而且FasL mRNA表达水平随夏枯草作用浓度增加显著上调,夏枯草不同浓度组比较均有显著性差异(P<0.01);随夏枯草作用浓度升高,SW480细胞侵袭能力明显增强,不同浓度组比较均有显著性差异(P<0.01)。结论:夏枯草在一定时间内均可上调人结肠癌SW480细胞FasL mRNA的表达,而且这种上调作用在一定范围内呈剂量依赖性,可使结肠癌细胞的侵袭能力增强。
Objective：To study the influence of the traditional Chinese medicine Prunella vulgaris on Fas Ligand mRNA expression of human colon cancer cell line SW480 and invasive ability. Methods： The inhibition concentration 50% （ IC50 ） of Prunella vulgaris was detected with M3T assay. Different concentrations（0.5IC50,IC50 ） of Prunella vulgaris was used in the cultured human colon cancer cell line SW480. The changes of Fas ligand mRNA were detected by the reverse transcription polymerase chain reaction method （ RT - PCR） and invasive abihty of SW480 cells was measured by Transwell - ECM. Results： RT - PCR results revealed that the higher concentration Prunella vulgaris upregulated Fas Ligand mRNA expression on SW480 cells （ P 〈 0.05 ） ; Transwell - ECM showed that the higher concentration Prunella vulgaris increased apoptosis rate of lymphocyte induced by SWd80 cells （ P 〈 0.05 ）. The comparisons of different concentrations qroups of Prunella vulgaris had statistical significance（ P 〈 0.05 ）. Experimental set up three groups, each repeated three sa/nples. Group A control group of 1640, B group Prunella 0.5 IC50 group,C group Prunella IC50 group. Conclusion： Prunella vulgaris can upregulate functional Fas ligand mRNA expression of human colon cancer cell line SW480. At the same time, the upregulative action can raise invasive ability of cancer cells.
出处
《现代肿瘤医学》
CAS
2009年第6期1034-1037,共4页
Journal of Modern Oncology
关键词
基因表达
夏枯草
结肠癌
侵袭
gene expression
Prunella vulgaris
colon cancer
invasion
