摘要
采用免疫分析对急性发作期支气管哮喘患儿、缓解期患儿及正常对照组血浆ＩＬ－２、ｓＩＬ－２Ｒ、ＩＬ－４、ＩＬ－５、ＩＬ－８、ＴＮＦα、ＩｇＥ等炎性介质水平进行测定；用逆转录聚合酶链反应技术（ＲＴ－ＰＣＲ）对外周淋巴细胞ＩＬ－４、ＩＬ－５ｍＲＮＡ表达进行定量分析。结果发作期哮喘患儿血浆ＩＬ－２、ｓＩＬ－２Ｒ、ＩＬ－４、ＩＬ－５、ＩＬ－８、ＴＮＦα和ＩｇＥ水平均明显高于正常对照组（Ｐ＜０．００１），以ＩＬ－４、ＩＬ－５和ＩｇＥ变化最为明显，缓解期均明显下降，但ＩＬ－４、ＩＬ－５及ＩｇＥ水平仍高于正常水平（Ｐ＜０．０１）。发作期哮喘患儿外周淋巴细胞ＩＬ－４、ＩＬ－５ｍＲＮＡ表达增强，治疗缓解后减弱，同时血浆ＩＬ－４、ＩＬ－５分别与ＩＬ－４、ＩＬ－５ｍＲＮＡ呈明显正相关关系（ｒ１＝０．６７，Ｐ＜０．００１；ｒ２＝０．７４，Ｐ＜０．００１）。结果：哮喘发作期血浆多种炎性介质水平显著升高、缓解后明显下降，提示非特异性炎性介质的释放与哮喘的发作密切相关。外周淋巴细胞ＩＬ－４、ＩＬ－５强表达以及血浆ＩＬ－４、ＩＬ－５高水平，提示哮喘发作期ＴＨ２亚群的激活；同时ＩＬ－８和ＴＮＦα的升高表明中性粒细胞和单核巨噬细胞可能参与了哮喘的?
o investigate the relationship between nonspecific inflammatory mediators and child bronchial asthma and to disclose the pathogenesis of asthma. Some inflammatory mediators such as IL2, sIL2R, IL4, IL5, IL8, TNFα and IgE in 64 children at attack stage, 45 at remission satge and 20 normal subjects have been measured with ELISA and radioimmunoassy, meanwhile IL4 and IL5 mRNA expression in lymphocytes were detected with RTPCR and PCR. The results showed that:1. All inflammatory mediators of children with severe asthma were significantly higher than those of normal controls(P<0001) and decreased apparently after treatment with glucocorticoid and β2agnosit, but IL4, IL5 and IgE still showed higher levels compared with those of controls;2. The expression of IL4 and IL5 mRNA in lymphocytes of acute asthmatic children were stronger than the other two groups(P<001), furthermore a positive relationship between plasma IL4,IL5 levels and IL4, IL5 mRNA expression were found respectively(r1=0.67, r2=0.74, P<0001). This study suggested that the raise of nonspecfic inflammatory mediators may be play an important role in pathogenosis and development of child asthma.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第3期224-226,共3页
Chinese Journal of Immunology
基金
南京市科委资助
