摘要
目的研究野菊花舒张血管及抗炎机制,同时探索其活性物质基础。方法通过离体血管环张力实验观察野菊花提取物及其主要单体对大鼠胸主动脉环经苯肾上腺素引起血管环收缩的影响。以Griess反应检测野菊花提取物及其单体对LPS(1μg/ml)/IFN-γ(10U/ml)刺激巨噬细胞RAW264.7释放NO的抑制作用;噻唑兰(MTT)比色法检测二者对细胞活力的影响;western blot检测二者对诱导型一氧化氮合酶(iNOS)及环氧合酶-2(COX-2)的抑制作用。结果野菊花提取物、木犀草素对血管的舒张效应为部分内皮依赖性、部分非内皮依赖性;二者对均能剂量依赖性地抑制炎症巨噬细胞生成NO、抑制iNOS表达,同时无细胞毒性,对COX-2表达无影响。结论野菊花提取物的血管舒张效应,抑制炎症细胞内NO及其诱导型合酶iNOS表达可能是其药理作用机制。
Objective To investigate the vasodilatory and anti-inflammatory mechanisms of Chrysanthemum indicum and its active components. Methods The major components of Chrysanthemum indicum extraction（CI） were detected by HPLC. The vasodilatoryeffects of CI and its active components were investigated by isometric tension changes of aortic rings. Griess and MTT assay were used to observe CI effects on iNOS expression and cell viability. The expression of iNOS and COX-2 was analyzed by western blot. Results Linarin and Luteolin were identified as two major components of CI. The vasedilatory effects of CI and luteolin were partially endothelium-dependent. CI and luteolin concentration dependently inhibited the LPS/IFN-γ-stimulated production of NO and iNOS without cell toxicity; however, the COX-2 expression was less affected by the compounds. Conclusion The effects of Cl and luteolin on vascular motion and inflammatory iNOS expression suggest the pharmcological mechanisms of Chrysanthemum indicum in traditional application; and luteolin is one of its important active components.
出处
《上海中医药杂志》
2009年第5期60-63,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
国家科技部"十一五"支撑计划(2006BAIⅡB08-03)
上海市教委高校一氧化氮与炎症医学E研究院计划(E-04010)
上海市科委国际技术转移专项(08430711300)
上海市科委中药现代化专线(08DZ1972104)
上海中医药大学科研资助项目
关键词
野菊花
一氧化氮
清热解毒
疏风平肝
Chrysanthemum indicum
nitric oxide（NO）
clearing heat and relieving toxin
dispersing wind and pacifying liver
