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α-硫辛酸拮抗铅对幼年大鼠骨生长及代谢的影响

α-lipoic acid antagonizes effects of lead on bone growth and metabolism in poisoned juvenile rats
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摘要 目的:探讨α-硫辛酸(LA)对染铅大鼠骨显微结构及骨髓生化指标的影响.方法:将SD大鼠随机分为2组,空白对照组和铅染毒组(230mg/L的醋酸铅灌胃),40d后各组取3只大鼠测血铅和骨铅,将已染铅大鼠随机分为4组,即铅对照组、3个LA治疗组[30,60,100mg/(kg.d)].LA治疗80d后处死动物,测定血铅、骨铅、HE染色观察骨小梁结构,免疫组化法检测碱性磷酸酶表达.结果:①LA各治疗组大鼠血铅、骨铅水平均低于铅对照组,差异有统计学意义(P<0.05).②LA100mg治疗组大鼠,HE染色观察骨小梁结构,碱性磷酸酶表达与铅对照组比较,差异有统计学意义(P<0.05).结论:LA能够拮抗铅的骨毒性. AIM: To determine the effects of α-lipoic acid on bone microstructure and bone marrow biochemical indicators in lead poisoned rats. METHODS: SD rats were divided into 2 groups: control group and lead group treated with lead acetate [ 230 ppm ]. After 40 d, 3 rats from each group were taken for the t of lead in blood and bone. The rats in lead group were then randomly subdivided into 4 groups : 1 lead control group and 3 lead groups with different concentrations of α-lipoic acid [30, 60 and 100 mg/(kg· d)]. At the end of the experiment (80 d), the rats were killed and the samples of blood and bone were taken for examination. RESULTS: Blood lead contents in every lead α-lipoic group were lower than those in lead control group (P 〈 0.05 ). HE staining showed that after treatment with α- lipoic acid, the bone microstructure and AKP immunity class brood in 100 mg lead α-lipoic acid group were significantly different from those in lead control group ( P 〈 0.05). CONCLUSION: α-lipoic acid antagonizes lead toxicity in bone marrow.
出处 《第四军医大学学报》 北大核心 2009年第11期993-996,共4页 Journal of the Fourth Military Medical University
基金 辽宁教育厅基金(2006T063 05L140)
关键词 Α-硫辛酸 骨/代谢 α-lipoic acid lead bone/metabolism
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参考文献13

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