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CTGF反义寡核苷酸对高糖培养人肾小球系膜细胞细胞外基质表达的影响 被引量：3

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摘要 目的:观察体外转染结缔组织生长因子(CTGF)反义寡核苷酸(ASODN)对高糖培养的人肾小球系膜细胞(HMC)细胞外基质表达的影响。方法:体外培养HMC,予以高糖(30mmol/L)和正常糖(5mmol/L)培养液培养,高糖培养后应用脂质体Lipofectamine2000转染CTGFASODN,在不同时间收集培养液上清。用ELISA检测培养液上清中的纤连蛋白(FN)和层黏连蛋白(LN)含量。结果:高糖呈时间依赖促进体外培养的HMC表达FN和LN,而转染CTGFASODN可抑制高糖所致的FN和LN分泌增加。结论:高糖培养可促进HMC细胞外基质的表达,转染CTGFASODN可抑制上述高糖的作用。故调控HMC的CTGF表达水平可能成为防治糖尿病肾病(DN)、特别是早期病变的有效途径之一。
作者 陈黎明 王秋月 吴丹
机构地区 中国医科大学附属一院内分泌科 沈阳医学院奉天医院皇姑屯医院内科 沈阳二四二医院干诊科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第6期556-558,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 辽宁省教育厅科学研究项目(202013140) 辽宁省自然科学基金资助项目(20032068)
关键词 结缔组织生长因子 反义寡核苷酸 细胞外基质 糖尿病肾病
分类号 R587.1 [医药卫生—内分泌]
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参考文献6

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  • 3赵龙,邓红,汪颖南,苏宁,刘必成,刘东风.高糖、茶多酚对人系膜细胞结缔组织生长因子、纤维连接蛋白mRNA表达的影响[J].南京医科大学学报（自然科学版）,2005,25(3):157-160. 被引量：5
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二级参考文献24

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细胞与分子免疫学杂志
2009年 第6期

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