摘要
本实验用抗纤维粘连蛋白(FN)亲和层析纯抗体和抗管蛋白抗体及鬼笔环肽,以免疫荧光组织化学方法,对NIH3T3细胞转染人肺腺癌细胞系AGZY基因组DNA前后细胞表面FN及细胞内骨架系统进行染色观察。结果表明,细胞在发生转化后,微丝及微管均表现出明显受损,细胞骨架结构不清,呈现为弥散样荧光;细胞表面FN大量减少,仅及正常NIH3T3细胞的1/9,其分布也由细丝形成的网状,变成斑点或斑块状。这一结果进一步证实,细胞恶变是涉及到细胞骨架系统及膜表面糖蛋白变化的复杂过程,并预示这些变化可能就是导致细胞形态发生变化、细胞失去正常生长调控的原因之一。
The experiment was designed to compare the changes of cytoskeleton (including tubulin and actin) and cell surface fibronectin (FN) between NIH 3T3 cell line and transformed NIH 3T3 cell line by genome DNA of human lung adenocarcinoma cell line AGZY. We stained and observed these cells using immunohistochemical methods with antibody against bovine brain tubulin, phalloidin and self-made affinity column purified antibody against porcine plasma FN.Our results showed that the bundles of actin and tubulin are damaged seriously, demonofrating an unclear cytoskeleton structure and diffused fluorescence over the cells when they were transformed. The amount of membrane FN on transformed cell surface decreases significantly which is only 1/9 of NIH 3T3. The FN distribution altered markedly from thin threadlike network to spots and speckles.These results suggested that cell transformation was a complex event including the changes of cytoskeleton system and cell surface glycoproteins. In addition, it might also indicate that cause and effect relationship existed between these changes and the alteration of cell phenotype and loss of growth control.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第3期265-268,共4页
Acta Anatomica Sinica
关键词
纤维粘连蛋白
细胞转化
免疫组化
Fibronectin (FN)
Tubulin
Actin
Immunohistochemistry
Phalloidin
Cell transformation
